Genome-derived Microsatellite Markers in Lippia origanoides from Colombia

IF 1.2 Q4 PHARMACOLOGY & PHARMACY

Journal of Herbs, Spices and Medicinal Plants Pub Date : 2022-09-15 DOI:10.1080/10496475.2022.2121351

Liliana Santamaría-Acevedo, M. M. Levy, L. Torres-Martínez, S. Marchant, Fernando Rondón-González, E. Stashenko, M. Levy, J. L. Fuentes

引用次数: 0

Abstract

ABSTRACT Polymorphic microsatellite sequences were mined from genome sequences of two Lippia origanoides wild specimens from disjunct locations. Twenty-three primer sequences flanking the candidate markers were designed, and their usefulness for population genetic analysis was assessed in plants sampled from four municipalities in Colombia. A final set of 10 primer pairs was useful for diversity analyses, which indicated the existence of three genetic lineages in Colombia. Cross-amplification in five related Lippia species was validated. The findings supported the efficiency of in silico approaches in providing novel genetic resources for research in Lippia species.

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哥伦比亚牛肝Lippia origanoides基因组微卫星标记研究

摘要/ ABSTRACT摘要:从两个不同地点的野鹅里皮(Lippia origanoides)野生标本的基因组序列中提取了多态微卫星序列。设计了候选标记侧翼的23个引物序列，并在哥伦比亚4个城市取样的植物中评估了它们在群体遗传分析中的有效性。最后一组10对引物对多样性分析有用，表明哥伦比亚存在3个遗传谱系。交叉扩增在5个亲缘Lippia种中得到验证。这一发现支持了计算机方法在为利皮亚物种研究提供新的遗传资源方面的有效性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

发文量

期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.