Expression of anthocyanin biosynthesis-related genes during flower development in Lilium spp.

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2022-09-01 DOI:10.1016/j.plgene.2022.100372

Hasan N.N. Fatihah , Katarzyna Wolinska , Jan G. Schaart , Marian Oortwijn , Richard G.F. Visser , Frans A. Krens

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引用次数: 1

Abstract

Sterility of hybrids produced from interspecific hybridization in lilies (Lilium, Liliaceae) is a great limitation in the breeding program, especially for Lilium longiflorum, which only has white-flowered cultivars. Because modification of flower colour in L. longiflorum by conventional breeding is limited by pre- and post-fertilization barriers, we think genetic modification could be used as an alternative in the future. For this, we need to understand what determines white colouration in L. longiflorum and other species and identify the molecular mechanisms regulating flower colour. In this study, we determined the accumulation of anthocyanins and related compounds in flower tissues during flower developmental stages in L. longiflorum cultivar ‘Lincoln’ and in the Oriental hybrid lily cultivars ‘Rialto’, ‘Perth’ and ‘Gran Tourismo’, respectively with white, pink and red flowers. Furthermore, the presence/absence and the expression of eight structural genes (CHSa, CHSb, CHIa, CHIb, F3H, F3’H, DFR, ANS) and three transcription factor genes (MYB12, MYB15, bHLH2) in flower tissues were investigated. Two structural genes (LlLinF3’H and LlLinDFR) and one transcription factor gene (LlLinbHLH2) were not detected in ‘Lincoln’ flowers. In ‘Rialto’, an amino acid substitution in the R2 repeat of LhRiaMYB12 which was previously reported to be responsible for the white flower colour is also found in the LhPerMYB12 of the pink ‘Perth’ flowers. Moreover, LhRiaDFR is present but not expressed in ‘Rialto’ flowers. Accumulation of cyanidin was observed in the flowers of ‘Perth’ and ‘Gran Tourismo’. High amounts of dihydrokaempferol accumulated in flowers of all four lily cultivars confirming the expression and functionality of early structural genes in the pathway. The elevated expression of the structural genes is strongly correlated with the expression of LhMYB12 and LhMYB15. This information can be used in the future to generate new L. longiflorum or Oriental lily hybrid cultivars with novel flower colours.

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百合花发育过程中花青素生物合成相关基因的表达。

百合(Lilium, Liliaceae)种间杂交产生的杂种不育性在育种中存在很大的局限性，特别是对于只有白花品种的长花百合(Lilium longiflorum)。由于传统育种方法对龙花的花色进行修饰受到受精前和受精后障碍的限制，我们认为转基因技术在未来可以作为一种替代方法。为此，我们需要了解长花L. longiflorum和其他物种的白色决定因素，并确定调节花色的分子机制。在本研究中，我们测定了L. longiflorum品种“Lincoln”和东方杂交百合品种“Rialto”、“Perth”和“Gran Tourismo”在花发育阶段花组织中花青素和相关化合物的积累情况，这些品种的花分别为白色、粉红色和红色。研究了8个结构基因(CHSa、CHSb、CHIa、CHIb、F3H、f3’h、DFR、ANS)和3个转录因子基因(MYB12、MYB15、bHLH2)在花组织中的表达情况。2个结构基因(lllinf3’h和lllinfr)和1个转录因子基因(LlLinbHLH2)在林肯花中未检测到。在' Rialto '中，LhRiaMYB12 R2重复序列中的氨基酸取代，之前报道过导致白色花朵的原因，也在粉红色' Perth '花的LhPerMYB12中发现。此外，LhRiaDFR在“Rialto”花中存在但不表达。在'珀斯'和'大旅游'的花中观察到花青素的积累。在所有四种百合品种的花中积累了大量的二氢山奈酚，证实了该途径中早期结构基因的表达和功能。结构基因的表达升高与LhMYB12和LhMYB15的表达密切相关。这些信息可用于今后培育具有新花色的长花百合或东方百合杂种新品种。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.