Vitamin D Activates Various Gene Expressions, Including Lipid Metabolism, in C2C12 Cells.

IF 1.1 4区医学 Q4 NUTRITION & DIETETICS

Journal of nutritional science and vitaminology Pub Date : 2022-02-28 DOI:10.3177/jnsv.68.65

Sako Mizutani, Mamoru Oyabu, Arisa Yamamoto, Ran Uchitomi, Takumi Sugimoto, Yasutomi Kamei

{"title":"Vitamin D Activates Various Gene Expressions, Including Lipid Metabolism, in C2C12 Cells.","authors":"Sako Mizutani, Mamoru Oyabu, Arisa Yamamoto, Ran Uchitomi, Takumi Sugimoto, Yasutomi Kamei","doi":"10.3177/jnsv.68.65","DOIUrl":null,"url":null,"abstract":"Vitamin D is a fat-soluble molecule, well known for its role in regulating calcium homeostasis in bone. It has become increasingly clear that it also has important effects in many other organs, including the skeletal muscle. In order to gain insight into the role of vitamin D in the skeletal muscle, we performed microarray analysis using C2C12 myoblasts treated with 1,25-dihydroxyvitamin D (1,25(OH)2D), active form of vitamin D. We found multiple genes upregulated by 1,25(OH)2D. Some of them, i.e., vitamin D receptor (Vdr), diacylglycerol O-acyltransferase (Dgat1 and Dgat2, the rate limiting steps of triacylglycerol acylation), and vascular endothelial growth factor A (Vegfa), were previously reported to be upregulated by 1,25(OH)2D in C2C12 cells. RT-qPCR analysis confirmed increased mRNA levels of Rarres2, Dio2, Tgm2, Lpl, Mdfi, Igfbp3, Dgat1, Crabp2, Gadd45a, Vagfa, Dgat2, C3, Ldhb, Cebpa, Igfbp5, Mrc2, Vdr. Thus, many genes, including lipid metabolism genes as well as genes related to muscle functions, appear to be upregulated by 1,25(OH)2D in muscle cells.","PeriodicalId":16624,"journal":{"name":"Journal of nutritional science and vitaminology","volume":"68 1 1","pages":"65-72"},"PeriodicalIF":1.1000,"publicationDate":"2022-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of nutritional science and vitaminology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3177/jnsv.68.65","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}

引用次数: 1

Abstract

Vitamin D is a fat-soluble molecule, well known for its role in regulating calcium homeostasis in bone. It has become increasingly clear that it also has important effects in many other organs, including the skeletal muscle. In order to gain insight into the role of vitamin D in the skeletal muscle, we performed microarray analysis using C2C12 myoblasts treated with 1,25-dihydroxyvitamin D (1,25(OH)2D), active form of vitamin D. We found multiple genes upregulated by 1,25(OH)2D. Some of them, i.e., vitamin D receptor (Vdr), diacylglycerol O-acyltransferase (Dgat1 and Dgat2, the rate limiting steps of triacylglycerol acylation), and vascular endothelial growth factor A (Vegfa), were previously reported to be upregulated by 1,25(OH)2D in C2C12 cells. RT-qPCR analysis confirmed increased mRNA levels of Rarres2, Dio2, Tgm2, Lpl, Mdfi, Igfbp3, Dgat1, Crabp2, Gadd45a, Vagfa, Dgat2, C3, Ldhb, Cebpa, Igfbp5, Mrc2, Vdr. Thus, many genes, including lipid metabolism genes as well as genes related to muscle functions, appear to be upregulated by 1,25(OH)2D in muscle cells.

查看原文本刊更多论文

维生素D激活C2C12细胞中的各种基因表达，包括脂质代谢。

维生素D是一种脂溶性分子，以其调节骨骼钙稳态的作用而闻名。越来越清楚的是，它对包括骨骼肌在内的许多其他器官也有重要影响。为了深入了解维生素D在骨骼肌中的作用，我们使用用维生素D的活性形式1,25-二羟基维生素D（1,25（OH）2D）处理的C2C12成肌细胞进行了微阵列分析。其中一些，即维生素D受体（Vdr）、二酰基甘油O-酰基转移酶（Dgat1和Dgat2，三酰基甘油酰化的限速步骤）和血管内皮生长因子A（Vegfa），先前报道在C2C12细胞中被1,25（OH）2D上调。RT-qPCR分析证实Rarres2、Dio2、Tgm2、Lpl、Mdfi、Igfbp3、Dgat1、Crabp2、Gadd45a、Vagfa、Dgat2、C3、Ldhb、Cebpa、Igfbp5、Mrc2、Vdr的mRNA水平增加。因此，许多基因，包括脂质代谢基因以及与肌肉功能相关的基因，似乎被肌肉细胞中的1,25（OH）2D上调。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of nutritional science and vitaminology 医学-营养学

CiteScore

1.80

自引率

6.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Nutritional Science and Vitaminology is an international medium publishing in English of original work in all branches of nutritional science, food science and vitaminology from any country. Manuscripts submitted for publication should be as concise as possible and must be based on the results of original research or of original interpretation of existing knowledge not previously published. Although data may have been reported, in part, in preliminary or abstract form, a full report of such research is unacceptable if it has been or will be submitted for consideration by another journal.