The Effect of Estrogen on Hepatic Fat Accumulation during Early Phase of Liver Regeneration after Partial Hepatectomy in Rats

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2019-08-27 DOI:10.1267/ahc.19018

Naparee Srisowanna, N. Choijookhuu, Koichi Yano, Baatarsuren Batmunkh, Makoto Ikenoue, Nguyen Nhat Huynh Mai, Y. Yamaguchi, Y. Hishikawa

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引用次数: 14

Abstract

Fatty liver is common in men and post-menopausal women, suggesting that estrogen may be involved in liver lipid metabolism. The aim of this study is to be clear the role of estrogen and estrogen receptor alpha (ERα) in fat accumulation during liver regeneration using the 70% partial hepatectomy (PHX) model in male, female, ovariectomized (OVX) and E2-treated OVX (OVX-E2) rats. Liver tissues were sampled at 0–48 hr after PHX and fat accumulation, fatty acid translocase (FAT/CD36), sterol regulatory element-binding protein (SREBP1c), peroxisome proliferator-activated receptor α (PPARα), proliferative cell nuclear antigen (PCNA) and ERα were examined by Oil Red O, qRT-PCR and immunohistochemistry, respectively. Hepatic fat accumulation was abundant in female and OVX-E2 compared to male and OVX rats. FAT/CD36 expression was observed in female, OVX and OVX-E2 at 0–12 hr after PHX, but not in male rats. At 0 hr, SREBP1c and PPARα were elevated in female and male rats, respectively, but were decreased after PHX in all rats. The PCNA labeling index reached a maximum at 36 hr and 48 hr in OVX-E2 and OVX rats, respectively. ERα expression in OVX-E2 was higher than OVX at 0–36 hr after PHX. In conclusion, these results indicated that estrogen and ERα might play an important role in fat accumulation related to FAT/CD36 during early phase of rat liver regeneration.

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雌激素对大鼠肝部分切除术后肝再生早期肝脏脂肪积累的影响

脂肪肝在男性和绝经后女性中很常见，这表明雌激素可能参与了肝脏的脂质代谢。本研究的目的是使用70%肝部分切除术（PHX）模型，在雄性、雌性、去卵巢（OVX）和E2处理的OVX（OVX-E2）大鼠中，明确雌激素和雌激素受体α（ERα）在肝脏再生过程中脂肪积累中的作用。PHX后0–48小时采集肝组织样本，分别用Oil Red O、qRT-PCR和免疫组织化学方法检测脂肪积累、脂肪酸转移酶（fat/CD36）、甾醇调节元件结合蛋白（SREBP1c）、过氧化物酶体增殖物激活受体α（PPARα）、增殖细胞核抗原（PCNA）和ERα。与雄性和OVX大鼠相比，雌性和OVX-E2大鼠的肝脏脂肪积累丰富。PHX后0–12小时，在雌性、OVX和OVX-E2中观察到FAT/CD36的表达，但在雄性大鼠中没有。在0小时时，雌性和雄性大鼠的SREBP1c和PPARα分别升高，但在PHX后所有大鼠均降低。PCNA标记指数在OVX-E2和OVX大鼠中分别在36小时和48小时达到最大值。在PHX后0-36小时，OVX-E2中ERα的表达高于OVX。总之，这些结果表明，雌激素和ERα可能在大鼠肝脏再生早期与fat/CD36相关的脂肪积累中发挥重要作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.