Potential of Streptomyces sp. in preventing the in vitro growth of Colletotrichum acutatum, the causative agent of infection in Capsicum annum L.

Rochmalia Juniarti Putri, R. Kawuri, A. Darmadi, I. Narayani
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Abstract

Red chilli plant (Capsicum annum L.) is one of the most popular vegetable crops in Indonesian society. One of them the pathogens attacks is Colletotrichum acutatum, a fungus causing anthracnose on red chilli. This study aims to determine the existence of Streptomyces sp. bacteria in the rhizosphere of the red chilli plant; the ability of Streptomyces sp. in inhibiting C. acutatum; Minimum Inhibitory Concentration (MIC) of Streptomyces isolates extracts in inhibiting C. acutatum; The Streptomyces isolation was carried out by dilution method using selective meida, namely Yeast Malt Agar. The Dual Culture method was used as an inhibition test between Streptomyces sp. and C. acutatum in vitro. A well diffusion method was used to test the effectiveness of the Streptomyces sp. and MIC filtrate concentration in inhibiting C. acutatum. The data obtained in this study were analyzed with Analysis of Varian (ANOVA) then continued with Duncan Multiple Range Test with 5% significance. Five Streptomyces isolates were found, namely Streptomyces sp.1, Streptomyces sp.2, Streptomyces sp.3, Streptomyces sp.4, and Streptomyces sp.5 in the rhizosphere of healthy C. annum L. plants in Daup Village, Kintamani District, Bangli Regency. Streptomyces sp. isolates. can significantly inhibit the growth of the fungus C. acuatum with inhibitory power ranging from 50.30% to 83.76%, Streptomyces sp.5 isolate was able to provide the highest percentage of inhibition in C. acutatum of 83.76 ± 2.91% with MIC 7% (v/v) with a diameter of 6.40 mm.
链霉菌(Streptomyces sp.)对辣椒炭疽病菌(Colletotrichum acutatum)体外生长的抑制作用。
红辣椒是印尼社会最受欢迎的蔬菜作物之一。其中一种病原体是尖锐炭疽菌,一种在红辣椒上引起炭疽病的真菌。本研究旨在确定红辣椒根际是否存在链霉菌属细菌;链霉菌(Streptomyces sp.)对尖尖孢梭菌(C.acutatum)的抑制能力;链霉菌分离物提取物对尖尖孢霉的最低抑菌浓度(MIC)研究;链霉菌的分离采用酵母麦芽琼脂稀释法进行。采用双培养法对尖柄链霉菌和链霉菌进行了体外抑菌试验。采用扩散法测定了链霉菌(Streptomyces sp.)和MIC滤液浓度对尖孢霉的抑制效果。本研究中获得的数据采用方差分析(ANOVA)进行分析,然后继续采用Duncan多范围检验,具有5%的显著性。在Bangli Regency Kintamani区Daup村的健康C.year L.植物根际发现了5个链霉菌分离株,即链霉菌1、链霉菌2、链霉菌3、链霉菌4和链霉菌5。链霉菌分离株。能显著抑制尖孢C.acutatum的生长,抑制率为50.30%~83.76%,链霉菌5株对尖孢C.aceutatum生长的抑制率最高,为83.76±2.91%,MIC为7%(v/v),直径为6.40mm。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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