Praziquantel and Moxidectin pharmacokinetics in dogs after Helmimax administration

Abstract

The purpose of the research is to study Praziquantel and Moxidectin pharmacokinetics in dogs after Helmimax administration.Materials and methods. Helmimax pharmacokinetics was studied on 8 adult male dogs of different breeds aged 2 to 5 years and weighing 15–35 kg. Helmimax was administered orally in the fasted state with a small amount of feed at a dose of 5 mg/kg for Praziquantel and 0.25 mg/kg for Moxidectin at the rate of 1 tablet per 10 kg of body weight. Blood was sampled at various periods after the administration. The collected blood underwent sample processing: formed element and protein precipitation, solid-phase extraction, and microfiltration. The active components were analyzed and detected by the HPLC-MS/MS. Active substances in the blood plasma were determined according to the developed technique which had been validated. The device was calibrated before the measurement.Results and discussion. As a result of the studies, the Praziquantel and Moxidectin pharmacokinetic parameters were calculated. The maximum concentration was 0.240 and 0.130 μg/mL, the time-to-peak concentration was 2.15 and 1.48 hours, and the elimination half-life was 8.41 and 3.61 hours for Moxidectin and Praziquantel, respectively.