Effects of Broccoli Extract (Brassica oleracea var. italica) on Collagen Images in Wounds: Experimental Study in Wistar Rats (Rattus norvegicus)

Abstract

ABSTRACTIntroduction: Wound healing is a protective function of the body that focuses on quick recovery where the regeneration process in a hostile environment takes more time. In particular, the oral cavity is a remarkable environment in which wound healing occurs in warm oral fluid that contains millions of microorganisms. Collagen plays an important role in all phases of wound healing, which provides integrity and strength to tissues and it is very important especially in the proliferation and remodeling phases. One of the materials that have potential related to collagen is broccoli (Brassica oleracea var.italica). Broccoli has a potential to regulate collagen, therefore it could be an alternative to answer the problem related to antiinflammatory, antioxidant, and antimicrobial effects. The aim of this research with experimental design is to study the potential effects of broccoli extract on the representation of collagen image in wounds.Methods: This research used 18 male wistar rats by making incision on each of the rats’ back. These rats are divided into two groups, control group which get no treatment, and treatment group which is treated by applying the broccoli extract topically twice a day. Wound tissues on each group are observed microscopically on 3th, 7th, and 14th day. The density of collagen fibers was measured based on the calculation of semiquantitative score. Result: The result showed that wound healing on treatment group tends to be better compared with control group with the increase of collagen score of treatment group. The result of Statistical test Man-Whitney with confidence level 95% (p < 0,05) with observation on 3th, 7th, and 14th day sequentally is  p=0.100, p=0,100, p=0,700. Conclusion: Broccoli extract which is applied topically tends to have more benefits regarding the process of wound healing, eventhough there is no significant difference found on statitistical test.