The effect of fish bone bioactive peptides on the wound healing process: an in vitro study on keratinocytes

Abstract

Fish bones mainly contain type I collagen and hydroxyapatite, and despite of their potential for applications in biotechnology and biomedicine, they represent one of the major source of waste generated by fish processing industry. The present study was focused on the interaction of bioactive peptides extracted from silver carp (H. molitrix) bones with human keratinocytes in culture. The potential of fish bone bioactive peptides to influence cell viability, proliferation and migration was evaluated in different experimental models in vitro. The results demonstrated a high efficiency and bioactivity of the enzymatically extracted fish bone peptides in several processes involved in cutaneous wound healing, in particular stimulation of keratinocytes metabolism and migration. In conclusion, they present a huge potential for applications in skin tissue engineering, but also in the biomedical and cosmetic fields.