Characterization of Titanium dioxide (TiO2 ) Nanoparticles Biosynthesized using Leuconostoc spp. Isolated from Cow’s Raw Milk

Q4 Agricultural and Biological Sciences
Susan Abdul, Raheem Hasan, Jehan Abdul Sattar, S. S. Al-jubori
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Abstract

Nanotechnology is a continually expanding field for its uses and applications in multiple areas i.e. medicine, science, and engineering. Biosynthesis is straightforward, less-toxicity, and cost-effective technology. TiO2 NPs biosynthesis has attained consideration in recent decades. In this study, probiotic bacteria were isolated from cow’s raw milk samples, and then were identified by using the Vitek2 system; as Leuconostoc spp. included Leuconostoc mesenteroides subsp. mesenteroides (Leu.1), Leuconostoc mesenteroides subsp. cremoris (Leu.4), and Leuconostoc pseudomesenteroides (Leu.14). All Leuconostoc spp. isolates showed an ability for TiO2 NPs bio-production, after being incubated at anaerobic conditions (30 o C/ 24 h) in DeMan Regosa and Sharpe (MRS) broth medium. The biosynthesized TiO2 nanoparticles were characterized using the following apparatuses: UV-Vis spectroscopy, X-ray diffraction (XRD) apparatus, Atomic Force Microscopy (AFM), Fourier Transform Infrared Spectroscopy (FTIR), Field Emission Scanning Electron Microscopy (FE-SEM) in addition to Energy Dispersive X-ray analysis (EDX) spectra. The characterized biosynthesized TiO2 NPs were spherical-shaped, nanostructure anatase crystals with an average size range of 53.35-59.41 nm. The UV absorption spectrum was observed at the wavelength 344-248 nm; the topography AFM 2D and 3D images result showed the height and roughness of biosynthesized TiO2 NPs that were in the range of 1.137-18.88 nm. Absorption peaks in the FTIR spectra were located in a region typical of TiO2 NPs, and biosynthesized TiO2 nanoparticles’ main IR topographies (408.21- 445.80) cm-1 belonged to anatase Titania (Ti-O-Ti) bridge.
从奶牛原料乳中分离的白垩白菌生物合成二氧化钛纳米颗粒的表征
纳米技术是一个不断扩大的领域,其用途和应用在多个领域,即医学,科学和工程。生物合成是一种直接、低毒、低成本的技术。近几十年来,TiO2纳米粒子的生物合成得到了广泛的关注。本研究从奶牛原料乳样品中分离出益生菌,并用Vitek2系统进行鉴定;如Leuconostoc sp.包括Leuconostoc mesenteroides亚sp.;肠系膜(Leu.1);cremoris (Leu.4)和Leuconostoc pseudomesenteroides (Leu.14)。在DeMan Regosa和Sharpe (MRS)肉汤培养基中厌氧(30℃/ 24 h)培养后,所有Leuconostoc菌株均表现出产TiO2 NPs的能力。利用紫外可见光谱、x射线衍射仪(XRD)、原子力显微镜(AFM)、傅里叶变换红外光谱(FTIR)、场发射扫描电镜(FE-SEM)以及能量色散x射线分析(EDX)等仪器对生物合成的TiO2纳米粒子进行了表征。所表征的生物合成TiO2 NPs为球形、纳米结构的锐钛矿晶体,平均尺寸范围为53.35 ~ 59.41 nm。紫外吸收光谱在344 ~ 248 nm处;形貌AFM二维和三维图像结果显示,生物合成的TiO2 NPs的高度和粗糙度在1.137 ~ 18.88 nm之间。FTIR光谱中的吸收峰位于TiO2纳米粒子的典型区域,生物合成的TiO2纳米粒子的主要红外形貌(408.21 ~ 445.80)cm-1属于锐钛矿型二氧化钛(Ti-O-Ti)桥。
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来源期刊
Proceedings of the Pakistan Academy of Sciences: Part B
Proceedings of the Pakistan Academy of Sciences: Part B Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
0.60
自引率
0.00%
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0
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