The Role and Mechanism of LncRNA-p21 in Regulating Gastric Cancer Metastasis by Mediating Wnt/β-Catenin Signaling Pathway

临床护理研究 Pub Date : 2023-05-31 DOI:10.26689/jcnr.v7i3.4862

Peng Liu, Haizhe Chang, Xinyu Peng, Xiuqin Zhang, Jianjun Zhang, Jiusong Luan

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Abstract

Objective: To study the mechanism of lncRNA p21 inhibiting the growth and metastasis of human gastric cancer SGC7901/GES-1 cells by mediating the Wnt/β-catenin signaling pathway. Methods: Lentiviral overexpression of lncRNA-p21 in human gastric cancer SGC7901/GES-1 cell transfections was observed and analyzed for in vitro migration, invasion, cell morphology and proliferation. Besides. Wnt/β-catenin signaling pathway was tested for direct involvement in lncRNA-p21-mediated inhibition of gastric cancer cell growth and proliferation. Wnt/β-catenin signaling pathway was validated using Li-C1. Results: Gastric cancer SGC7901/GES-1 cells in the overexpression of lncRNA-p21 showed changes in stellate morphology, low invasion, or spindle-shaped morphology. LncRNA-p21 inhibited the growth and proliferation of gastric cancer SGC7901/GES-1 cells both in vivo and in vitro, and Wnt/β-catenin signaling pathway mediated the proliferation, invasion, and migration of lncRNA-p21 on gastric cancer SGC7901/GES-1 cells. Conclusion: LncRNA-p21 can inhibit the growth and metastasis of gastric cancer SGC7901/GES-1 cells in vitro and in vivo, and the inhibition of lncRNA p21 is mainly mediated by inhibiting the Wnt/β-catenin signaling pathway.

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LncRNA-p21介导Wnt/β-Catenin信号通路调控胃癌转移的作用及机制

目的：探讨lncRNAp21介导Wnt/β-catenin信号通路抑制人癌症SGC7901/GES-1细胞生长转移的机制。方法：观察lncRNA-p21慢病毒在人癌症SGC7901/GES-1细胞转染中的过度表达，并分析其体外迁移、侵袭、细胞形态和增殖。此外检测Wnt/β-catenin信号通路直接参与lncRNA-p21-介导的对癌症细胞生长和增殖的抑制。利用Li-C1对Wnt/β-catenin信号通路进行了验证。结果：癌症SGC7901/GES-1细胞在lncRNA-p21的过度表达中表现出星状形态、低侵袭或棘状形态的改变。LncRNA-p21在体内外均能抑制癌症SGC7901/GES-1细胞的生长和增殖，Wnt/β-catenin信号通路介导LncRNA-p21对癌症SGC7901-GES-1细胞增殖、侵袭和迁移。结论：LncRNA-p21在体内外均可抑制癌症SGC7901/GES-1细胞的生长和转移，其抑制LncRNA-p21的作用主要通过抑制Wnt/β-catenin信号通路来实现。

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临床护理研究

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