Ecotype variations in karyotypes and 2C DNA content in Drimia indica (Roxb.) Jossop: An important medicinal plant

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2023-07-26 DOI:10.1016/j.jarmap.2023.100506

Padmaja Patel , Surbat Kumar Dehery , Satya Narayan Jena , Chinmay Pradhan , Anath Bandhu Das

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Abstract

Indian squill (Drimia indica) belonging to the family Asparagaceae is a highly medicinal herb with several therapeutic properties. Detailed karyotype analysis and 2C DNA contents were reported in twelve ecotypes of D. indica from different geographical locations in the state of Odisha, India. The diploid somatic chromosome 2n = 20 was recorded in four ecotypes ODi-16 (Kendrapada), ODi-23 (Tikarpada), ODi-24 (Daspalla), and ODi-26 (Odagaon) while tetraploid having 2n = 40 was newly reported in one ecotype from Nuagaon (Eco-ODi-06). Aneuploid chromosome number 2n = 26 was obtained in ecotypes from Similipal and Satkosia besides 2n = 32 was newly reported in ecotypes from Bitarkanika and Narasinghpur. The ecotypes ODi-22 (Nayagarh) and ODi-11 (Nilagiri) were found to be with 2n = 16 and 2n = 22 respectively. The 2C DNA content analysis showed a 2.19-fold increase which is confirmed by an increase in the number and chromosome length of ecotypes. The 2C DNA values were found highest at 36.26 p.g. (2n = 40) in Eco-ODi-06 (Nuagaon) and lowest 16.54 p.g. (2n = 16) in Eco-ODi-22 of Nayagarh that corresponds with ploidy and genome size. The asymmetry in the karyotypes showed non-significant clusters among the ecotypes except ecotypes ODi-26 (Odagaon) and ODi-2a (Bhitarkanika) which are most asymmetric having advanced karyotypic features as compared to the ecotypes ODi-01, ODi-08 and ODi-22 having symmetric karyotypes. The chromosome characteristics along with genome size analysis of D. indica can be very useful in establishing genome-specific cytotypes which can be used for breeding programs for crop improvement. Furthermore, enhancement of therapeutic phytoconstituents and cyto-taxonomic identification from adulterants and confirmation of allopolyploidy or autopolyploidy through genomic in situ hybridization could unravel the potential of this plant in relation to secondary metabolite production.

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籼稻核型和2c DNA含量的生态型变异一种重要的药用植物

印度松鼠属于天冬科，是一种高度药用的草药，具有多种治疗特性。报道了来自印度奥里萨邦不同地理位置的12个生态型籼稻的核型分析和2C DNA含量。在肯德拉帕达(Kendrapada)、提卡尔帕达(Tikarpada)、达斯帕拉(Daspalla)、Odagaon (Odagaon) 4个生态型中分别发现了2n = 20的二倍体体细胞染色体，在Nuagaon (ecoodi -06)中发现了2n = 40的四倍体体细胞染色体。除Bitarkanika和Narasinghpur新报道的2n = 32染色体外，Similipal和Satkosia生态型的非整倍体染色体数为2n = 26。ODi-22 (Nayagarh)和ODi-11 (Nilagiri)生态型分别为2n = 16和2n = 22。2C DNA含量增加了2.19倍，这与生态型数量和染色体长度的增加有关。与倍性和基因组大小相对应，Nuagaon的Eco-ODi-06的2C DNA值最高，为36.26 p.g. (2n = 40)， Nayagarh的Eco-ODi-22的2C DNA值最低，为16.54 p.g. (2n = 16)。除ODi-26 (Odagaon)和ODi-2a (Bhitarkanika)外，其余生态型的核型不对称性不显著，其核型特征较ODi-01、ODi-08和ODi-22更为先进。对籼稻染色体特征和基因组大小的分析，可为籼稻基因组特异性细胞型的建立提供参考，为作物改良育种提供依据。此外，加强治疗性植物成分和对掺假物的细胞分类鉴定，以及通过基因组原位杂交确认异源多倍体或自多倍体，可以揭示该植物与次生代谢物生产有关的潜力。

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来源期刊

Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

6.40

自引率

7.70%

发文量

审稿时长

41 days

期刊介绍： JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.