Molecular detection and identification of a ‘Candidatus Phytoplasma solani’-related strain associated with pumpkin witches’ broom in Xinjiang, China

IF 1.9 3区农林科学 Q2 AGRONOMY

Phytopathologia Mediterranea Pub Date : 2021-05-13 DOI:10.36253/PHYTO-11066

Xu Wang, Chun-guang Wang, Xiao-Yan Li, Zheng Li

引用次数: 0

Abstract

Pumpkin plants showing symptoms of witches’ broom (PuWB) were observed in Xinjiang Uyghur autonomous region, China, in September 2018. A phytoplasma was detected in symptomatic plants by PCR amplifying portions of the 16S ribosomal and tuf genes. In addition, the phylogeny based on these genes sequencing indicated that the PuWB strain clusters with ‘Candidatus Phytoplasma solani’ (subgroup 16SrXII-A). Furthermore, based on in silico and in vitro restriction fragment length polymorphism analyses, the PuWB phytoplasma was confirmed as a ‘Ca. P. solani’-related strain. This was the first record of the occurrence of phytoplasma presence in pumpkins in China, and the first record of 16SrXII phytoplasma infecting pumpkins in the world.

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一株与新疆南瓜扫帚相关的“Candidatus PhytopPlasma solani”菌株的分子检测与鉴定

2018年9月，中国新疆维吾尔自治区的南瓜出现了“女巫扫帚病”症状。通过PCR扩增16S核糖体和tuf基因部分，在有症状的植株中检测到植物原体。此外，基于这些基因测序的系统发育表明，PuWB菌株与候选植物solani (Candidatus Phytoplasma) (16SrXII-A亚群)属一类。此外，基于芯片和体外限制性片段长度多态性分析，PuWB植物原体被证实为“Ca”。solani的相关菌株。这是中国第一次记录到植物原体在南瓜中存在，也是世界上第一次记录到16SrXII植物原体侵染南瓜。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytopathologia Mediterranea 生物-植物科学

CiteScore

4.40

自引率

8.30%

发文量

审稿时长

6-12 weeks

期刊介绍： Phytopathologia Mediterranea is an international journal edited by the Mediterranean Phytopathological Union. The journal’s mission is the promotion of plant health for Mediterranean crops, climate and regions, safe food production, and the transfer of new knowledge on plant diseases and their sustainable management. The journal deals with all areas of plant pathology, including etiology, epidemiology, disease control, biochemical and physiological aspects, and utilization of molecular technologies. All types of plant pathogens are covered, including fungi, oomycetes, nematodes, protozoa, bacteria, phytoplasmas, viruses, and viroids. The journal also gives a special attention to research on mycotoxins, biological and integrated management of plant diseases, and the use of natural substances in disease and weed control. The journal focuses on pathology of Mediterranean crops grown throughout the world. The Editorial Board of Phytopathologia Mediterranea has recently been reorganised, under two Editors-in-Chief and with an increased number of editors.