Mechanism of the effect of Xiaoyao powder treatment on exercise capacity of depressed rats—A stable isotope tracer metabolomic study

IF 1 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of Liquid Chromatography & Related Technologies Pub Date : 2022-07-21 DOI:10.1080/10826076.2022.2163499

Cui Ji, Weidi Zhao, Jie Zheng, Shi Zhou, Jun-sheng Tian, Yumei Han, Xuemei Qin

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引用次数: 2

Abstract

Abstract Depression accounts for 10% of the total global burden of nonfatal disease. Xiaoyao Powder, a well-known traditional Chinese medicine is commonly used to treat depression. However, its therapeutic mechanisms have not yet been fully elucidated. In this study, a rat depression model was established by the application of chronic unpredictable mild stress (CUMS). The depressed rats were randomly distributed to four groups: Blank Control, CUMS control, CUMS with Venlafaxine (a positive control) treatment, and CUMS with Xiaoyao Powder treatment, with 12 rats in each group. Depression behavioral and exercise tests were performed weekly during the four-week intervention. Stable isotope tracer metabolomics and molecular biology techniques were utilized to determine the changes in serum metabolites pre and post the intervention period, aiming to elucidate the mechanism of Xiaoyao Powder in improving the depression symptoms and exercise capacity of the depressed rats. The results found that the Xiaoyao Powder treatment significantly improved the performance in the behavioral and exercise tests, as well as affected the levels of metabolites, such as pyruvic acid, lactic acid, and citric acid in serum. It was concluded that Xiaoyao Powder intervention had a significant effect on the pyruvic acid metabolism, glycolysis/gluconeogenesis, and tricarboxylic acid cycle in the serum of the depressed rats, which could contribute to the understanding of the mechanism of the intervention. GRAPHICAL ABSTRACT

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逍遥散对抑郁大鼠运动能力影响的机制——稳定同位素示踪代谢组学研究

摘要抑郁症占全球非致命疾病总负担的10%。逍遥散是一种著名的中药，常用于治疗抑郁症。然而，其治疗机制尚未完全阐明。本研究采用慢性不可预测轻度应激（CUMS）建立大鼠抑郁模型。将抑郁大鼠随机分为空白对照组、CUMS对照组、文拉法辛治疗组和逍遥散治疗组，每组12只。在为期四周的干预期间，每周进行抑郁行为和运动测试。采用稳定同位素示踪代谢组学和分子生物学技术，测定干预前后血清代谢产物的变化，旨在阐明逍遥散改善抑郁大鼠抑郁症状和运动能力的机制。结果发现，逍遥散治疗显著改善了行为和运动测试的表现，并影响了血清中丙酮酸、乳酸和柠檬酸等代谢产物的水平。结论逍遥散干预对抑郁大鼠血清丙酮酸代谢、糖酵解/糖异生和三羧酸循环有显著影响，有助于了解干预机制。图形摘要

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来源期刊

Journal of Liquid Chromatography & Related Technologies 化学-分析化学

CiteScore

2.80

自引率

0.00%

发文量

审稿时长

4.9 months

期刊介绍： The Journal of Liquid Chromatography & Related Technologies is an internationally acclaimed forum for fast publication of critical, peer reviewed manuscripts dealing with analytical, preparative and process scale liquid chromatography and all of its related technologies, including TLC, capillary electrophoresis, capillary electrochromatography, supercritical fluid chromatography and extraction, field-flow technologies, affinity, and much more. New separation methodologies are added when they are developed. Papers dealing with research and development results, as well as critical reviews of important technologies, are published in the Journal.