First Report of Cucurbit Aphid-Borne Yellows Virus Infecting Groundnut in Kenya

IF 1.7 Q2 PLANT SCIENCES

Plant Health Progress Pub Date : 2022-10-31 DOI:10.1094/php-08-22-0071-rs

A. Mabele, F. Muyekho, H. Wéré

{"title":"First Report of Cucurbit Aphid-Borne Yellows Virus Infecting Groundnut in Kenya","authors":"A. Mabele, F. Muyekho, H. Wéré","doi":"10.1094/php-08-22-0071-rs","DOIUrl":null,"url":null,"abstract":"Cucurbit aphid-borne yellows virus (CABYV, genus Polerovirus, family Luteoviridae) causing cucurbit aphid-borne yellows disease (CABYD) in groundnut (Arachis hypogaea), is characterized by interveinal yellowing symptoms in leaves. CABYV is transmitted by Aphis gossypii, Myzus persicae and Macrosiphum euphorbiae. CABYV causes up to 80% yield loss but there is no information available on its occurrence, serological and molecular characteristics in groundnuts. Two disease diagnostic surveys were conducted during the short and long rain seasons of 2020 and 2021 in Bungoma, Busia, Kakamega and Siaya counties growing groundnut. Disease incidence and severity was scored and data collected analysed using R Software. Serological bioassays were done using TAS-ELISA and positive samples pooled then processed with the transposon-based chemistry library preparation kit (Nextera XT, Illumina). Phylogenetic analyses and comparisons were performed using the MEGA X software. The short rains season recorded higher incidence (73.61%) than the long rains season (42.65%). The CABYV isolates from Kenya clustered with other poleroviruses variedly. CABYV6-2 showed 94.5% nucleotide identity with CpPV2 isolate KX599164.1 from Burkina Faso and 83.4% identity with CABYV isolate MG257902.1 from Korea. This research provides evidence for the first report of CABYV infecting A. hypogaea relevant for development of better plant health management technologies for increased groundnut yield.","PeriodicalId":20251,"journal":{"name":"Plant Health Progress","volume":" ","pages":""},"PeriodicalIF":1.7000,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Health Progress","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1094/php-08-22-0071-rs","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 1

Abstract

Cucurbit aphid-borne yellows virus (CABYV, genus Polerovirus, family Luteoviridae) causing cucurbit aphid-borne yellows disease (CABYD) in groundnut (Arachis hypogaea), is characterized by interveinal yellowing symptoms in leaves. CABYV is transmitted by Aphis gossypii, Myzus persicae and Macrosiphum euphorbiae. CABYV causes up to 80% yield loss but there is no information available on its occurrence, serological and molecular characteristics in groundnuts. Two disease diagnostic surveys were conducted during the short and long rain seasons of 2020 and 2021 in Bungoma, Busia, Kakamega and Siaya counties growing groundnut. Disease incidence and severity was scored and data collected analysed using R Software. Serological bioassays were done using TAS-ELISA and positive samples pooled then processed with the transposon-based chemistry library preparation kit (Nextera XT, Illumina). Phylogenetic analyses and comparisons were performed using the MEGA X software. The short rains season recorded higher incidence (73.61%) than the long rains season (42.65%). The CABYV isolates from Kenya clustered with other poleroviruses variedly. CABYV6-2 showed 94.5% nucleotide identity with CpPV2 isolate KX599164.1 from Burkina Faso and 83.4% identity with CABYV isolate MG257902.1 from Korea. This research provides evidence for the first report of CABYV infecting A. hypogaea relevant for development of better plant health management technologies for increased groundnut yield.

查看原文本刊更多论文

肯尼亚首次报道南瓜蚜传黄病毒感染花生

南瓜蚜传黄病毒（CABYV，Polerovirus属，黄病毒科）引起花生（Arachis hypogaea）的瓜蚜传黄病（CABYD），其特征是叶片叶脉间发黄。CABYV由棉蚜、桃蚜和大戟传播。CABYV导致高达80%的产量损失，但没有关于其在花生中的发生、血清学和分子特征的信息。在2020年和2021年的短雨季和长雨季，在种植花生的本戈马、布西亚、卡卡梅加和西亚县进行了两次疾病诊断调查。对疾病发生率和严重程度进行评分，并使用R软件对收集的数据进行分析。使用TAS-ELISA进行血清学生物测定，将阳性样本合并，然后用基于转座子的化学文库制备试剂盒（Nextera XT，Illumina）处理。使用MEGA X软件进行系统发育分析和比较。短雨季的发病率（73.61%）高于长雨季（42.65%）。来自肯尼亚的CABYV分离株与其他波兰病毒不同程度地聚集在一起。CABYV6-2与来自布基纳法索的CpPV2分离株KX599164.1显示94.5%的核苷酸同一性，与来自韩国的CABYV分离株MG257902.1显示83.4%的同一性。这项研究为首次报道CABYV感染深生花生提供了证据，该报告与开发更好的植物健康管理技术以提高花生产量有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plant Health Progress Agricultural and Biological Sciences-Horticulture

CiteScore

3.00

自引率

13.00%

发文量

期刊介绍： Plant Health Progress, a member journal of the Plant Management Network, is a multidisciplinary science-based journal covering all aspects of applied plant health management in agriculture and horticulture. Both peer-reviewed and fully citable, the journal is a credible online-only publication. Plant Health Progress is a not-for-profit collaborative endeavor of the plant health community at large, serving practitioners worldwide. Its primary goal is to provide a comprehensive one-stop Internet resource for plant health information.