Construction of high density genetic map and quantitative trait locus mapping in a sorghum Tx623A × sudangrass S722 population

IF 1.1 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Grassland Science Pub Date : 2022-12-04 DOI:10.1111/grs.12394

Wenmiao Tu, Lihua Wang, Peng Jin, Ruirui Meng, Jian Zheng, Yanlong Liu, Yi-Hong Wang, Jieqin Li

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引用次数: 0

Abstract

Sorghum–sudanense is a hybrid of sorghum and Sudanese grass and is widely used in animal husbandry and aquaculture because of its high yield, excellent quality and wide adaptability. Using high-throughput sequencing, we constructed a high-density genetic map of sorghum Tx623A × sudangrass S722 with 103 recombinant inbred lines (RILs) in this study and mapped quantitative trait loci (QTLs) for plant height (PH), stem diameter (SD), tiller number (TN), fresh weight (FW) and dry weight per plant (DW). The map included 1610 markers with a total length of 2329 cM and QTL mapping identified 19 QTLs, including two for PH, three for SD, four for TN, four for FW and five for DW. We detected two QTL clusters containing overlapping QTLs for PH, FW and DW. Orthologs of three SD candidate genes from maize were found within two SD QTLs. The identification of these QTLs will be useful for cloning the corresponding genes for these traits and developing marker-assisted selection (MAS) in future sorghum breeding programs.

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高粱Tx623A ×苏丹草S722群体高密度遗传图谱构建及数量性状位点定位

苏丹高粱是高粱与苏丹草的杂交品种，因其产量高、品质优良、适应性广而广泛应用于畜牧业和水产养殖业。利用高通量测序技术，构建了含有103个重组自交系(ril)的高粱Tx623A ×苏丹草S722的高密度遗传图谱，并绘制了单株株高(PH)、茎粗(SD)、分蘖数(TN)、鲜重(FW)和干重(DW)的数量性状位点(qtl)。该图谱包含1610个标记，总长度为2329 cM，共鉴定出19个QTL，其中PH 2个，SD 3个，TN 4个，FW 4个，DW 5个。我们检测到了PH、FW和DW三个QTL重叠的两个QTL簇。在2个玉米SD qtl中发现了3个候选基因的同源物。这些qtl的鉴定将为今后高粱育种中相应性状基因的克隆和标记辅助选择(MAS)的开展提供依据。

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来源期刊

Grassland Science Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

>12 weeks

期刊介绍： Grassland Science is the official English language journal of the Japanese Society of Grassland Science. It publishes original research papers, review articles and short reports in all aspects of grassland science, with an aim of presenting and sharing knowledge, ideas and philosophies on better management and use of grasslands, forage crops and turf plants for both agricultural and non-agricultural purposes across the world. Contributions from anyone, non-members as well as members, are welcome in any of the following fields: grassland environment, landscape, ecology and systems analysis; pasture and lawn establishment, management and cultivation; grassland utilization, animal management, behavior, nutrition and production; forage conservation, processing, storage, utilization and nutritive value; physiology, morphology, pathology and entomology of plants; breeding and genetics; physicochemical property of soil, soil animals and microorganisms and plant nutrition; economics in grassland systems.