Effects of dietary avocado meal on production performance, carcass traits, muscle fatty acid composition and gene expression of pigs

IF 0.5 4区农林科学 Q4 VETERINARY SCIENCES

Veterinaria Mexico Pub Date : 2022-10-04 DOI:10.22201/fmvz.24486760e.2022.1017

C. Lemus-Flores, Job Oswaldo Bugarín Prado, Fernando Grageola Núñez, Karina Mejía Martínez, Roberto Valdivia Bernal, J. S. Segura Correa

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引用次数: 0

Abstract

Dietary avocado meal (AM) may improve growth and muscle fatty acid composition in pigs due to the expression of genes. Transcriptome analysis of Longissimus dorsi muscle and liver and the effect of 0, 5 and 10 % AM in the feed of Landrace-Yorkshire pigs on growth, carcass, muscle fatty acid composition and blood metabolites were evaluated. Twenty-four castrated males with an initial weight of 55 ± 3 kg were distributed under a completely random design. Transcriptome analysis was carried out with massive sequencing, with EdgeR and DESeq2 methods for differentially gene expression (DGE; P ≤ 0.01, log2 fold change ≤ 1.5). We observed higher DGE in both tissues, with down log2 fold change, by including 5 or 10 % AM in the diet against AM0. Genes associated with lipid metabolism (down ANO3; up GOS2, MYLIP, PLIN4), growth (down FOS, MT1A, MT1D, PHGDH; up ARNTL) circadian clock (down DBP, NOCT, PER1, PER2, PER3, SIK1; up ARNTL), immune system (down CD163, CRP, OTUD1; up C1QTNF7, NFIL3) and antioxidative activity (up HP and NCF4) were observed. The inclusion of AM in the diets increased daily gain, feed intake, slaughtered body weight, carcass weight and ham (P < 0.05). Back fat was greater for AM10 (P < 0.001). AM in the diet decreased intramuscular fat and had less triglycerides in the blood (P < 0.05). AM10 in the diet modified the gene expression and quality of the IMF, had more linoleic acid, total fatty acids Ω6, ∑polyunsaturated fatty acids, PUFA/SFA, and PUFA/MUFA (P < 0.001)

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日粮牛油果粉对猪生产性能、胴体性状、肌肉脂肪酸组成及基因表达的影响

由于基因的表达，日粮鳄梨粉（AM）可以改善猪的生长和肌肉脂肪酸组成。对约克郡长白猪背最长肌和肝脏的转录组分析以及0、5和10%AM对生长、胴体、肌肉脂肪酸组成和血液代谢产物的影响进行了评价。在完全随机的设计下，24名初始体重为55±3 kg的阉割雄性被分配。转录组分析采用大规模测序、EdgeR和DESeq2方法进行差异基因表达（DGE；P≤0.01，log2倍变化≤1.5）。我们观察到，在针对AM0的饮食中加入5%或10%AM，这两种组织的DGE较高，log2倍数变化较低。观察到与脂质代谢（ANO3下降；GOS2、MYLIP、PLIN4上升）、生长（FOS、MT1A、MT1D、PHGDH下降；ARNTL上升）昼夜节律时钟（DBP、NOCT、PER1、PER2、PER3、SIK1下降；ARNTL上升）、免疫系统（CD163、CRP、OTUD1下降；C1QTNF7、NFIL3上升）和抗氧化活性（HP和NCF4上升）相关的基因。日粮中加入AM增加了日增重、采食量、屠宰体重、胴体重量和火腿（P<0.05）。AM10的背部脂肪更大（P<0.001）。日粮中的AM减少了肌肉内脂肪，血液中的甘油三酯更少（P<0.05），∑多不饱和脂肪酸、PUFA/SFA和PUFA/MUFA（P＜0.001）

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来源期刊

Veterinaria Mexico Veterinary-General Veterinary

CiteScore

1.60

自引率

0.00%

发文量

期刊介绍： Veterinaria México OA (ISSN 2448-6760) is an online scientific journal edited by Universidad Nacional Autónoma de México (UNAM). The journal is Open Access and follows UNAM''s initiative, to transmit knowledge free of charge to the readership and authors, with no Article Processing Charges. This journal publishes advances in Veterinary Sciences and Animal Production, and to reach more lectures across the world the journal was updated since 2014 from its predecessor printed in paper Veterinaria México (ISSN 0301-5092) and its digital version (ISSN 2007-5472).