Genetic improvement of traditional Basmati rice Ranbir Basmati for semi-dwarfism and blast resistance through molecular breeding

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2022-12-01 DOI:10.1016/j.plgene.2022.100386

Tushar Diliprao Pote , Anish Kaachra , Kalpna Thakur , Ramesh Kumar Salgotra , S. Gopala Krishnan , Rajeev Rathour

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引用次数: 1

Abstract

The traditional Basmati rices grown in north-western foothills of Himalayas in India are known world-wide for their exquisite aroma and exceptional eating and cooking qualities. These varieties are tall and suffer heavy losses due to their susceptibility to lodging as well as blast disease. In present study, we improved a popular traditional Basmati rice variety ‘Ranbir Basmati’ for semi-dwarfism and resistance to blast by incorporating the semi-dwarfing gene, sd1 and two blast resistance genes, Pi9 and Pi54 through marker-assisted backcross breeding. Combining marker-assisted background selection with stringent phenotypic selection for aroma and seed quality traits during backcrossing enabled faster recovery of recurrent parent genome and reconstitution of the genomic regions associated with Basmati quality traits in the gene pyramided lines. All pyramided lines were significantly short statured with a reduction of 23.46 to 40.26 cm in height, as compared to recurrent parent, with many of them exhibiting higher grain yield and superior quality attributes than Ranbir Basmati. The yield superiority of the pyramided lines primarily resulted from the improvement in panicle number and semi-dwarf stature conferred by sd1 gene. The gene pyramided lines showed complete resistance to five highly virulent blast races that collectively showed compatibility with 16 different resistance genes, suggesting the broad-spectrum resistance in these lines. The semi-dwarf, high yielding and blast resistant lines generated herein are potential candidates for release as cultivars for overcoming losses in Ranbir Basmati due to lodging and blast disease.

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传统巴斯马蒂稻兰比尔半矮化和抗稻瘟病的分子育种遗传改良

传统的印度香米生长在印度喜马拉雅山脉的西北山麓，以其精致的香气和独特的食用和烹饪品质而闻名于世。这些品种高大，由于易倒伏和枯萎病，损失惨重。本研究利用半矮化基因sd1和两个抗稻瘟病基因Pi9和Pi54，通过标记辅助回交育种，对传统巴斯马蒂稻品种“兰比尔巴斯马蒂”进行了半矮化和抗稻瘟病的改良。在回交过程中，将标记辅助背景选择与香气和种子品质性状的严格表型选择相结合，可以更快地恢复回交亲本基因组，并在基因金字塔系中重建与巴斯马蒂品质性状相关的基因组区域。所有的金字塔系与循环亲本相比，高度降低了23.46 ~ 40.26 cm，显著缩短，且许多金字塔系表现出比兰比尔巴斯马蒂更高的产量和品质特征。锥体系的产量优势主要来自于sd1基因在穗数和半矮秆高度上的提高。基因金字塔系对5个高毒力的瘟病小种表现出完全的抗性，这些小种与16种不同的抗性基因共表现出相容性，表明这些系具有广谱抗性。由此产生的半矮秆、高产和抗稻瘟病品系是克服兰比尔巴斯马蒂因倒伏和稻瘟病造成的损失的潜在候选品种。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.