Detection of Glanders in horses of eight Iraqi provinces by ELISA

Abstract

The present study was carried out to determine the prevalence of Glanders in horses of Iraq. For this reason, a serological surveillance was conducted during the period extended between November 2015 and March 2016. Four hundred and four samples of serum were collected from horses lived in eight different Iraqi provinces that were Al-Muthanna, Baghdad, Al-Najaf, Babylon, Al-Qadsyia, Diyala, Karbala and Wasit. Sera transported to the lab of clinical pathology/ department of internal and preventive veterinary medicine at Baghdad University, where they were analyzed by cELISA technique to determine the seropositivity against Burkholderia mallei. The results of sera analysis showed that 2.97 % (12 horses) were positive toward the applied test. The positive cases were recorded in Baghdad and Muthanna governorate (11 and 1, respectively). Statistical analysis revealed no significant variations (P<0.05) in the seropositivity against Glanders between different genders or ages.