Hevea brasiliensis latex dialysed C-serum precipitate subfraction exerts a negligible level of genotoxicity in the Ames test, mouse lymphoma assay and micronucleus assay

IF 1.2 4区化学 Q4 POLYMER SCIENCE

Journal of Rubber Research Pub Date : 2023-05-05 DOI:10.1007/s42464-023-00201-x

Malini Devi Raman, Norazreen Abd Rahman, Sunderasan Elumalai, Vikneswaran Murugaiyah, Ming Thong Ong

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Abstract

Dialysed C-serum precipitate (DCP), a sub-fraction of latex C-serum originating from Hevea brasiliensis, has shown specific in vitro antiproliferative properties towards cancer-origin cell lines. As a potential agent to be used in cancer treatment, preclinical safety assessment tests are crucial. This study focused on investigating the mutagenicity of DCP via bacterial reverse mutation assay (Ames test), Mouse lymphoma assay (MLA), and micronucleus assay according to OECD guidelines. No mutagenicity was exhibited by DCP at concentrations lower than 1 mg/mL for the five different auxotroph strains of Salmonella typhimurium (TA 98, TA 100, TA 1535, and TA 1537) and Escherichia coli WP2uvrA pKM101 in the absence and presence of metabolic activation system (S9-mix). Nonetheless, it has been found that DCP was able to induce negligible mutation in MLA and micronucleus assay. Overall, DCP does express dose-dependant mutagenicity according to the assays conducted and thus, it is crucial to take this into consideration should DCP be used in the development of therapeutic agent.

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在Ames试验、小鼠淋巴瘤试验和微核试验中，巴西橡胶树胶乳透析的c -血清沉淀亚组分的遗传毒性可忽略不计

透析c -血清沉淀(DCP)是源自巴西橡胶树(Hevea brasiliensis)的乳胶c -血清的一个亚组分，已显示出对癌症起源细胞系的特异性体外抗增殖特性。作为一种潜在的用于癌症治疗的药物，临床前安全性评估试验至关重要。本研究主要通过细菌反向突变试验(Ames试验)、小鼠淋巴瘤试验(MLA)和微核试验研究DCP的致突变性。浓度低于1 mg/mL的DCP对5种营养不良的鼠伤寒沙门菌(ta98、ta100、ta1535和ta1537)和大肠杆菌WP2uvrA pKM101在代谢激活系统(S9-mix)缺失和存在的情况下均无致突变性。尽管如此，已经发现DCP能够在MLA和微核试验中诱导可忽略不计的突变。总的来说，根据所进行的试验，DCP确实表现出剂量依赖性的致突变性，因此，如果在治疗剂的开发中使用DCP，考虑到这一点是至关重要的。

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来源期刊

Journal of Rubber Research 化学-高分子科学

自引率

15.40%

发文量

审稿时长

3 months

期刊介绍： The Journal of Rubber Research is devoted to both natural and synthetic rubbers, as well as to related disciplines. The scope of the journal encompasses all aspects of rubber from the core disciplines of biology, physics and chemistry, as well as economics. As a specialised field, rubber science includes within its niche a vast potential of innovative and value-added research areas yet to be explored. This peer reviewed publication focuses on the results of active experimental research and authoritative reviews on all aspects of rubber science. The Journal of Rubber Research welcomes research on: the upstream, including crop management, crop improvement and protection, and biotechnology; the midstream, including processing and effluent management; the downstream, including rubber engineering and product design, advanced rubber technology, latex science and technology, and chemistry and materials exploratory; economics, including the economics of rubber production, consumption, and market analysis. The Journal of Rubber Research serves to build a collective knowledge base while communicating information and validating the quality of research within the discipline, and bringing together work from experts in rubber science and related disciplines. Scientists in both academia and industry involved in researching and working with all aspects of rubber will find this journal to be both source of information and a gateway for their own publications.