A new, ecological and stability-indicating method by HPLC for the quantification of moxifloxacin in tablets

Abstract

A new, ecological, and stability-indicating method by HPLC was developed for the quantification of moxifloxacin in tablets. A C18 column at 33°C, purified water acidified with 0.2% phosphoric acid and ethanol (74:26,v/v), flow rate at 1.0 mLmin-1, injection volume of 5 µL, and detection at 290 nm was used. Moxifloxacin (MOX), a fluoroquinolone antimicrobial, does not present a physical-chemical analytical method for tablets form described in an official compendium. On the other hand, the literature shows some methods for evaluating MOX by HPLC. However, they bring opportunities for improvement. The method was linear (2-12 μgmL-1), selective, accurate (100.86%), precise (RSD<2%), and robust. The retention time for moxifloxacin in tablets was approximately 4.4 minutes. A Inertsil ODS-4 (150 x 4.6 mm, 5 μm, GL SciencesTM) column at 33 °C, purified water acidified with 0.2 % phosphoric acid and ethanol (74: 26, v/v) as mobile phase, flow rate at 1.0 mL min-1, injection volume of 5 µL and detection at 290 nm were used. To sum up, a method based on the principles of green analytical chemistry was successfully developed and validated for the quantification of moxifloxacin in tablets. Keywords: HPLC, green analytical chemistry, method validation, tablets, fluoroquinolone, moxifloxacin.