Contribution to Pharmacological Valorisation of Algerian Arctium Minus (Hill) Bernh. Subsp. Atlanticum (Pomel) Maire; Antioxidant and Acetylcholinesterase Inhibitory Activities.

Q4 Pharmacology, Toxicology and Pharmaceutics
Sofiane Guettaf, Abdelmoumen Benmerzoug, Bensouici Chawki, Y. Cakmak, S. Dahamna, A. Baghiani, D. Harzallah
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引用次数: 0

Abstract

The traditional pharmacopoeia is full of potential resources for molecules with therapeutic effects involving the inhibition of enzymes linked to some diseases. This work aimed to test invitro the neuroprotective activity against Alzheimer's disease (AD) combined with the antioxidant effect of root extracts obtained by water, water / methanol and ethyl acetate of the endemic Arctium minus. subsp. atlanticum. native of Algeria. The different extracts of the root of the studied plant were obtained by decoction or maceration. The inhibitory effect of acetyl / butyryl cholinesterase (AChE/BChE) was achieved by a colorimetric method. Similarly, the antioxidant activity was measured, based on several mechanisms: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) and galvinoxyl (GOR) radicals free scavenging assays, β-carotene bleaching inhibition activity, and cupric ion reducing antioxidant capacity (CUPRAC). In the various tests carried out, the ethyl acetate extract (EAE) possessed the most powerful antioxidant and anticholinesterase activities in comparison with the other samples. The IC50 and A0.5 values of DPPH, GOR, β-carotene, CUPRAC, anti-AChE and anti BChE assays were 69.45±5.49, 28.87±0.18, 121.58±16.76, 37.41±1.59, 265±21 and 240±6.3 µg / mL, respectively. Likewise, a correlation can be deduced between the type of extract and the potent antioxidant / anticholinesterase potential. This work evokes for the first time the anticholinesterase potential combined with the antioxidant effect of Algerian Arctium minus. subsp. atlanticum. This association between the two effects could be effective in the fight against AD and therefore, the use of this natural resource may be possible in combating this aspect of neurodegeneration.
阿尔及利亚牛蒡子(Hill) Bernh)药理价值的贡献。无性系种群。大西洋(波梅尔)梅尔;抗氧化和乙酰胆碱酯酶抑制活性。
传统药典中充满了具有治疗作用的分子的潜在资源,包括抑制与某些疾病相关的酶。本研究旨在体外测试牛蒡根水、水/甲醇和乙酸乙酯提取液对阿尔茨海默病(AD)的神经保护作用和抗氧化作用。无性系种群。atlanticum。阿尔及利亚人。采用煎煮法和浸渍法分别从所研究的植物的根中提取不同的提取物。用比色法测定了乙酰/丁基胆碱酯酶(AChE/BChE)的抑制作用。同样,基于几种机制测量了抗氧化活性:1,1 -二苯基-2-癸基-肼(DPPH)和galvinoxyl (GOR)自由基清除试验,β-胡萝卜素漂白抑制活性和铜离子还原抗氧化能力(CUPRAC)。在各种试验中,与其他样品相比,乙酸乙酯提取物(EAE)具有最强的抗氧化和抗胆碱酯酶活性。DPPH、GOR、β-胡萝卜素、CUPRAC、抗ache和抗BChE的IC50和A0.5分别为69.45±5.49、28.87±0.18、121.58±16.76、37.41±1.59、265±21和240±6.3µg / mL。同样,可以推断出提取物类型与有效的抗氧化/抗胆碱酯酶潜能之间的相关性。这项工作首次唤起了抗胆碱酯酶潜能与阿尔及利亚牛蒡负的抗氧化作用的结合。无性系种群。atlanticum。这两种作用之间的联系可能在对抗阿尔茨海默病中有效,因此,利用这种自然资源可能在对抗神经退行性疾病方面发挥作用。
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来源期刊
Current Enzyme Inhibition
Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
1.30
自引率
0.00%
发文量
30
期刊介绍: Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.
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