In vitro Antioxidant, α-amylase and Horseradish Peroxidase Inhibitory Potential of Phenolics Extracts from Chamomilla pubescens, Pulicaria crispa and Rhanterium adpressum growing in Algeria

Q4 Pharmacology, Toxicology and Pharmaceutics
Feriel Mahfoudi, M. Reguia, M. Harrat, A. Djeridane, M. Yousfi
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引用次数: 1

Abstract

Plants are a main source of drugs for the therapy of a large number of diseases. The aim of the present work is to evaluate the in vitro antioxidant and anti-α-amylase and anti-peroxidases (HPR) potentials of phenolic extracts obtained from three spontaneous plants; growing in the South of Algeria such as (Chamomilla pubescens, Pulicaria crispa, and Rhanterium adpressum). This is the first report on the study of α-amylase and horseradish peroxidase (HRP) inhibitory activity for phenolic extracts from the Chamomilla pubescens and Pulicaria crispa plants. The antioxidant activity was evaluated in vitro using four tests: DPPH, CUPRAC, FRAP, and ABTS. The phenolic, flavonoid, and tannin compounds of the three selected Algerian plants were quantified. Also, the inhibition of α-amylase and HRP was evaluated. The quantification of the total phenolic contents revealed that they are widely variable, and depending on extraction solvents, the highest content was recorded by the ethyl acetate extract of Chamomilla pubescens (flowers) 774.93±60.14mg/100 g of dry matter. In all the antioxidant tests, ethyl acetate extracts showed the most effective activity, which the best was (VCEAC = 65.62 ±0.50 µM/g dry matter) of Pulicaria crispa for the DPPH test. Furthermore, the results of α-amylase and peroxidase inhibitory effects indicated that all plants extracts have inhibitory effects on the two enzymes, with AEIC values ranged from 76.55±3.54 to 149.54±6.68 μM/g of dry matter for the α-amylase, and CEIC values ranged from 8.89±2.22 to 9668.31±254.42 μM/g of dry matter for the peroxidase (HRP). The present study results suggest that the three Algerian spontaneous plant species (Rhanterium adpressum, Pulicaria crispa, and Chamomilla pubescens) inhibit peroxidase and α-amylase and exhibit a high antioxidant activity what can be related to the treatment of diabetes and thyroid diseases.
阿尔及利亚产洋甘菊、毛缕菊和苦参酚类物质提取物的体外抗氧化、α-淀粉酶和辣根过氧化物酶抑制潜力
植物是治疗许多疾病的主要药物来源。研究了三种天然植物酚类提取物的体外抗氧化、抗α-淀粉酶和抗过氧化物酶(HPR)活性;生长在阿尔及利亚南部的一种植物,如洋甘菊(Chamomilla pubescens)、洋甘菊(Pulicaria crispa)和洋甘菊(Rhanterium adpressum)。本文首次报道了洋甘菊和金缕菊酚类提取物对α-淀粉酶和辣根过氧化物酶(HRP)抑制活性的研究。采用DPPH、CUPRAC、FRAP和ABTS四项试验评价其体外抗氧化活性。对三种阿尔及利亚植物的酚类、类黄酮和单宁化合物进行了定量分析。同时对α-淀粉酶和HRP的抑制作用进行了评价。总酚含量的测定结果表明,总酚含量变化很大,不同提取溶剂对总酚含量的影响最大,洋甘菊(花)乙酸乙酯提取物的总酚含量为774.93±60.14mg/100 g(干物质)。在所有抗氧化试验中,乙酸乙酯提取物表现出最有效的抗氧化活性,其中对DPPH的活性最好的是白头翁(VCEAC = 65.62±0.50µM/g干物质)。此外,α-淀粉酶和过氧化物酶抑制结果表明,所有植物提取物对α-淀粉酶和过氧化物酶均有抑制作用,α-淀粉酶的AEIC值为76.55±3.54 ~ 149.54±6.68 μM/g,过氧化物酶(HRP)的CEIC值为8.89±2.22 ~ 9668.31±254.42 μM/g。本研究结果表明,阿尔及利亚三种天然植物(Rhanterium adpressum, Pulicaria crispa和Chamomilla pubescens)抑制过氧化物酶和α-淀粉酶,并表现出较高的抗氧化活性,可能与治疗糖尿病和甲状腺疾病有关。
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来源期刊
Current Enzyme Inhibition
Current Enzyme Inhibition Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
1.30
自引率
0.00%
发文量
30
期刊介绍: Current Enzyme Inhibition aims to publish all the latest and outstanding developments in enzyme inhibition studies with regards to the mechanisms of inhibitory processes of enzymes, recognition of active sites, and the discovery of agonists and antagonists, leading to the design and development of new drugs of significant therapeutic value. Each issue contains a series of timely, in-depth reviews written by leaders in the field, covering a range of enzymes that can be exploited for drug development. Current Enzyme Inhibition is an essential journal for every pharmaceutical and medicinal chemist who wishes to have up-to-date knowledge about each and every development in the study of enzyme inhibition.
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