Evidence for the association between FTO gene variants and vitamin B12 concentrations in an Asian Indian population.

IF 3.3 3区医学 Q2 GENETICS & HEREDITY

Genes and Nutrition Pub Date : 2019-09-05 eCollection Date: 2019-01-01 DOI:10.1186/s12263-019-0649-3

Shelini Surendran, Ramamoorthy Jayashri, Lauren Drysdale, Dhanasekaran Bodhini, Nagarajan Lakshmipriya, Coimbatore Subramanian Shanthi Rani, Vasudevan Sudha, Julie A Lovegrove, Ranjit M Anjana, Viswanathan Mohan, Venkatesan Radha, Rajendra Pradeepa, Karani S Vimaleswaran

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引用次数: 0

Abstract

Background: Low vitamin B12 concentrations have been associated with major clinical outcomes, including adiposity, in Indian populations. The Fat mass and obesity-associated gene (FTO) is an established obesity-susceptibility locus; however, it remains unknown whether it influences vitamin B12 status. Hence, we investigated the association of two previously studied FTO polymorphisms with vitamin B12 concentrations and metabolic disease-related outcomes and examined whether these associations were modified by dietary factors and physical activity.

Methods: A total of 176 individuals with type 2 diabetes, 152 with pre-diabetes, and 220 normal glucose-tolerant individuals were randomly selected from the Chennai Urban Rural Epidemiology Study. Anthropometric, clinical, and biochemical investigations, which included body mass index (BMI), waist circumference, vitamin B12, homocysteine, and folic acid were measured. A validated food frequency questionnaire was used for dietary assessment and self-reported physical activity measures were collected. An unweighted genetic risk score (GRS) was calculated for two FTO single-nucleotide polymorphisms (rs8050136 and rs2388405) by summation of the number of risk alleles for obesity. Interaction analyses were performed by including the interaction terms in the regression model.

Results: The GRS was significantly associated with increased BMI (P = 0.009) and risk of obesity (P = 0.023). Individuals carrying more than one risk allele for the GRS had 13.13% lower vitamin B12 concentrations, compared to individuals carrying zero risk alleles (P = 0.018). No associations between the GRS and folic acid and homocysteine concentrations were observed. Furthermore, no statistically significant GRS-diet or GRS-physical activity interactions with vitamin B12, folic acid, homocysteine or metabolic-disease outcomes were observed.

Conclusion: The study shows for the first time that a genetic risk score using two FTO SNPs is associated with lower vitamin B12 concentrations; however, we did not identify any evidence for the influence of lifestyle factors on this association. Further replication studies in larger cohorts are warranted to investigate the association between the GRS and vitamin B12 concentrations.

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亚洲印度人群中FTO基因变异与维生素B12浓度之间关系的证据

背景：在印度人群中，维生素 B12 浓度低与肥胖等主要临床结果有关。脂肪量和肥胖相关基因（FTO）是一个已确定的肥胖易感基因位点；然而，它是否会影响维生素 B12 的状态仍是未知数。因此，我们研究了之前研究过的两种 FTO 多态性与维生素 B12 浓度和代谢性疾病相关结果的关系，并考察了这些关系是否会因饮食因素和体育锻炼而改变：从钦奈城乡流行病学研究中随机选取了176名2型糖尿病患者、152名糖尿病前期患者和220名葡萄糖耐量正常者。他们接受了人体测量、临床和生化检查，包括体重指数（BMI）、腰围、维生素 B12、同型半胱氨酸和叶酸。膳食评估采用了有效的食物频率问卷，并收集了自我报告的体力活动量。通过对两个 FTO 单核苷酸多态性（rs8050136 和 rs2388405）的肥胖风险等位基因数求和，计算出了非加权遗传风险评分（GRS）。在回归模型中加入交互项进行交互分析：结果：GRS 与体重指数（BMI）的增加（P = 0.009）和肥胖风险（P = 0.023）明显相关。与携带零风险等位基因的个体相比，携带一个以上GRS风险等位基因的个体维生素B12浓度低13.13%（P = 0.018）。没有观察到 GRS 与叶酸和同型半胱氨酸浓度之间存在关联。此外，没有观察到GRS-饮食或GRS-体育锻炼与维生素B12、叶酸、同型半胱氨酸或代谢性疾病结果之间有统计学意义的相互作用：该研究首次表明，使用两个 FTO SNPs 的遗传风险评分与维生素 B12 浓度较低有关；但是，我们没有发现任何证据表明生活方式因素对这种关联有影响。有必要在更大的队列中开展进一步的重复研究，以调查 GRS 与维生素 B12 浓度之间的关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Genes and Nutrition 生物-遗传学

CiteScore

6.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： This journal examines the relationship between genetics and nutrition, with the ultimate goal of improving human health. It publishes original research articles and review articles on preclinical research data coming largely from animal, cell culture and other experimental models as well as critical evaluations of human experimental data to help deliver products with medically proven use.