Characterization of the atypical Meloidogyne arenaria (Tylenchina: Meloidogynidae) in Japan

IF 1.3 4区农林科学 Q2 ENTOMOLOGY

Applied Entomology and Zoology Pub Date : 2023-05-11 DOI:10.1007/s13355-023-00828-7

Gaku Murata, Toshihisa Yashiro, Taketo Uehara, Kenta Uesugi, Hideaki Iwahori, Takashi Narabu

{"title":"Characterization of the atypical Meloidogyne arenaria (Tylenchina: Meloidogynidae) in Japan","authors":"Gaku Murata, Toshihisa Yashiro, Taketo Uehara, Kenta Uesugi, Hideaki Iwahori, Takashi Narabu","doi":"10.1007/s13355-023-00828-7","DOIUrl":null,"url":null,"abstract":"<div>Genus Meloidogyne parasitizes and causes galling on host roots with heavy yield losses. Meloidogyne arenaria (Neal 1889) Chitwood 1949, one of the major species, is found in Japan and can be divided into two genotypes: M. arenaria A2-J and A2-O. Whereas the latter is typical M. arenaria, the former is empirically considered as atypical morphologically and genetically, but its detailed characteristics and phylogenetic relationships have not been described. First, we examined the perineal pattern, isozyme pattern, and PCR products with the C2F3/1108 primer set of M. arenaria A2-J and confirmed that all the populations of M. arenaria A2-J used in this study showed M. javanica and/or M. incognita -like perineal patterns, the A2 pattern in esterase isozyme, and approx. 1.7 kb amplicon. We then carried out a phylogenetic analysis based on the sequences of a partial region of COXII-IrDNA in mitochondrial DNA to reveal the relationships between M. arenaria A2-J and related species using corresponding sequences of Meloidogyne accessions deposited in GenBank. Moreover, host suitability of representative Solanaceae plants including Solanum torvum was evaluated and compared to those of major species of Meloidogyne collected from Japan.\n</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 3","pages":"245 - 255"},"PeriodicalIF":1.3000,"publicationDate":"2023-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s13355-023-00828-7.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Entomology and Zoology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13355-023-00828-7","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Genus Meloidogyne parasitizes and causes galling on host roots with heavy yield losses. Meloidogyne arenaria (Neal 1889) Chitwood 1949, one of the major species, is found in Japan and can be divided into two genotypes: M. arenaria A2-J and A2-O. Whereas the latter is typical M. arenaria, the former is empirically considered as atypical morphologically and genetically, but its detailed characteristics and phylogenetic relationships have not been described. First, we examined the perineal pattern, isozyme pattern, and PCR products with the C2F3/1108 primer set of M. arenaria A2-J and confirmed that all the populations of M. arenaria A2-J used in this study showed M. javanica and/or M. incognita -like perineal patterns, the A2 pattern in esterase isozyme, and approx. 1.7 kb amplicon. We then carried out a phylogenetic analysis based on the sequences of a partial region of COXII-IrDNA in mitochondrial DNA to reveal the relationships between M. arenaria A2-J and related species using corresponding sequences of Meloidogyne accessions deposited in GenBank. Moreover, host suitability of representative Solanaceae plants including Solanum torvum was evaluated and compared to those of major species of Meloidogyne collected from Japan.

Abstract Image

查看原文本刊更多论文

日本非典型竞技场根结线虫的特征（Tylenchina:根结线虫科）

Meloidogyne属寄生在寄主的根上，造成严重的产量损失。赤木1949 (Chitwood 1949)是在日本发现的主要种之一，可分为赤木A2-J和赤木A2-O两种基因型。后者是典型的沙粒支原体，而前者在形态学和遗传学上被认为是非典型的，但其详细特征和系统发育关系尚未被描述。首先，我们用C2F3/1108引物组检测了沙场螺旋藻A2- j的会阴模式、同工酶模式和PCR产物，证实本研究中所有沙场螺旋藻A2- j群体均表现为javanica和/或M. incognita样会阴模式、酯酶同工酶A2模式，以及近似于其他类群的会阴模式。1.7 kb放大器。基于线粒体DNA中COXII-IrDNA部分区域的序列进行系统发育分析，利用GenBank中保存的Meloidogyne资料的相应序列揭示了m.a arenaria A2-J与近缘种之间的关系。此外，还对茄科代表性植物(包括托鲁茄)的寄主适宜性进行了评价，并与日本主要种茄的寄主适宜性进行了比较。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Applied Entomology and Zoology 生物-昆虫学

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.