Vegetative propagation of Berberis asiatica Roxb. Ex DC., Ginkgo biloba L., Rauvolfia serpentina (L.) Benth ex Kurz, and Rhododendron arboreum Sm. through stem cuttings

IF 3.8 2区农林科学 Q1 PLANT SCIENCES

Journal of Applied Research on Medicinal and Aromatic Plants Pub Date : 2023-09-01 DOI:10.1016/j.jarmap.2023.100509

L.S. Lodhiyal , Neelu Lodhiyal , Riya Gupta , Sushma Tamta , Falak Siddiqui , Harsh Kumar Chauhan

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Abstract

The population of medicinal plants such as Berberis asiatica, Ginkgo biloba, Rauvolfia serpentina, and Rhododendron arboreum is declining from their natural habitats due to high commercial exploitation, medicinal value, slow-growing nature, and climate change. The present study was carried to assess the effects of IAA, IBA, and Rootex PD.01 (commercial 1000 mg kg⁻¹ of indole-3-butyric acid formulation) on survival and growth performance of stem cuttings so as to develop the vegetative propagation protocols for B. asiatica, G. biloba, R. serpentina, and R. arboreum. Four hundred eighty cuttings (120 cuttings of each species) with 0.5–1 cm diameter and 10 cm length were sterilized with 1 g L⁻¹ bavistin and treated with 250 mg L⁻¹ concentration of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) and Rootex PD.01 (1000 mg kg-1 of IBA) respectively. The survival was highest in G. biloba (90 %) followed by R. serpentina (83.3 %), B. asiatica (80 %) and R. arboreum (66.6 %). Stem cuttings treated with Rootex PD.01 exhibited maximum root length in species i.e. B. asiatica (5.2 cm), G. biloba (8.3 cm), R. serpentina (8.4 cm) and R. arboreum (6.7 cm) respectively. Other growth parameters such as shoot height, shoot diameter, number of leaves per node, number of leaves per plant, and root number were also maximum in stem cuttings treated with Rootex PD.01. This study concluded Rootex PD.01 for the propagation of these species thereby ensuring inexpensive and efficient protocols for mass-scale cultivation.

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亚洲小檗无性繁殖的研究。前女友。银杏，银杏叶，银杏叶库尔兹和杜鹃花。钻杆钻屑

由于商业开发程度高、药用价值高、生长缓慢以及气候变化等原因，亚洲小檗、银杏、蛇尾、杜鹃花等药用植物的数量正逐渐减少。本研究旨在研究IAA、IBA和Rootex PD.01 (1000 mg kg - 1吲哚-3-丁酸商业配方)对柠条扦插成活率和生长性能的影响，以制定柠条扦插成活率和生长性能的无性繁殖方案。以直径0.5-1 cm、长10 cm的扦插480根(每种120根)，分别用1 g L−1的巴伐丁素灭菌，并用250 mg L−1浓度的IAA(吲哚-3-乙酸)、IBA(吲哚-3-丁酸)和Rootex PD.01 (1000 mg kg-1的IBA)处理。钉螺成活率最高，为90%，其次为蛇形钉螺(83.3%)、亚洲钉螺(80%)和木林钉螺(66.6%)。经Rootex PD.01处理后的茎条根长最大，分别为亚洲木犀(B. asiatica) (5.2 cm)、双叶木犀(G. biloba) (8.3 cm)、蛇形木犀(R. serpentina) (8.4 cm)和木犀(R. arboreum) (6.7 cm)。根茎插条的茎高、茎粗、节叶数、单株叶数、根数等其他生长参数在Rootex PD.01处理下均达到最大值。本研究确定了Rootex PD.01用于这些物种的繁殖，从而确保了廉价和高效的大规模栽培方案。

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来源期刊

Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

6.40

自引率

7.70%

发文量

审稿时长

41 days

期刊介绍： JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.