Duplication of spiralian-specific TALE genes and evolution of the blastomere specification mechanism in the bivalve lineage

IF 4.1 2区生物学 Q1 DEVELOPMENTAL BIOLOGY

Evodevo Pub Date : 2021-07-23 DOI:10.21203/RS.3.RS-733292/V1

Supanat Phuangphong, Jumpei Tsunoda, H. Wada, Yoshiaki Morino

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引用次数: 3

Abstract

Background Despite the conserved pattern of the cell-fate map among spiralians, bivalves display several modified characteristics during their early development, including early specification of the D blastomere by the cytoplasmic content, as well as the distinctive fate of the 2d blastomere. However, it is unclear what changes in gene regulatory mechanisms led to such changes in cell specification patterns. Spiralian-TALE (SPILE) genes are a group of spiralian-specific transcription factors that play a role in specifying blastomere cell fates during early development in limpets. We hypothesised that the expansion of SPILE gene repertoires influenced the evolution of the specification pattern of blastomere cell fates. Results We performed a transcriptome analysis of early development in the purplish bifurcate mussel and identified 13 SPILE genes. Phylogenetic analysis of the SPILE gene in molluscs suggested that duplications of SPILE genes occurred in the bivalve lineage. We examined the expression patterns of the SPILE gene in mussels and found that some SPILE genes were expressed in quartet-specific patterns, as observed in limpets. Furthermore, we found that several SPILE genes that had undergone gene duplication were specifically expressed in the D quadrant, C and D quadrants or the 2d blastomere. These expression patterns were distinct from the expression patterns of SPILE in their limpet counterparts. Conclusions These results suggest that, in addition to their ancestral role in quartet specification, certain SPILE genes in mussels contribute to the specification of the C and D quadrants. We suggest that the expansion of SPILE genes in the bivalve lineage contributed to the evolution of a unique cell fate specification pattern in bivalves.

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双壳系中螺旋体特异性TALE基因的重复和卵裂球规范机制的进化

背景尽管螺旋纲动物的细胞命运图是保守的，但双壳类动物在其早期发育过程中表现出一些改变的特征，包括细胞质含量对D卵裂球的早期指定，以及2d卵裂球独特的命运。然而，目前尚不清楚是什么基因调控机制的变化导致了细胞规格模式的变化。螺旋体TALE（SPILE）基因是一组螺旋体特异性转录因子，在帽贝早期发育过程中，在指定卵裂球细胞命运方面发挥作用。我们假设SPILE基因库的扩增影响了卵裂球细胞命运的特定模式的进化。结果我们对紫色分叉贻贝的早期发育进行了转录组分析，并鉴定了13个SPILE基因。对软体动物SPILE基因的系统发育分析表明，SPILE基因重复发生在双壳类谱系中。我们检测了SPILE基因在贻贝中的表达模式，发现一些SPILE基因以四重特异性模式表达，如在帽贝中观察到的。此外，我们发现几个经过基因复制的SPILE基因在D象限、C象限和D象限或2d卵裂球中特异性表达。这些表达模式与SPILE在帽贝中的表达模式不同。结论这些结果表明，除了它们在四象限规范中的祖先作用外，贻贝中的某些SPILE基因也有助于C和D象限的规范。我们认为，SPILE基因在双壳类谱系中的扩展有助于双壳类独特细胞命运规范模式的进化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Evodevo EVOLUTIONARY BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

7.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： EvoDevo publishes articles on a broad range of topics associated with the translation of genotype to phenotype in a phylogenetic context. Understanding the history of life, the evolution of novelty and the generation of form, whether through embryogenesis, budding, or regeneration are amongst the greatest challenges in biology. We support the understanding of these processes through the many complementary approaches that characterize the field of evo-devo. The focus of the journal is on research that promotes understanding of the pattern and process of morphological evolution. All articles that fulfill this aim will be welcome, in particular: evolution of pattern; formation comparative gene function/expression; life history evolution; homology and character evolution; comparative genomics; phylogenetics and palaeontology