Involvement of NUCLEOPORIN1 in cell division and expansion in Arabidopsis

IF 2.2 Q3 GENETICS & HEREDITY

Plant Gene Pub Date : 2022-12-01 DOI:10.1016/j.plgene.2022.100385

Raj Kumar Thapa , Gang Tian , Xin Xie , Susanne E. Kohalmi , Yuhai Cui

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引用次数: 2

Abstract

NUCLEOPORIN1 (NUP1), a component of the nuclear pore complex and an anchor for the TREX-2 mRNA export complex, was previously reported to have diverse functions in Arabidopsis. Several studies have shown that mutations in NUP1 lead to small stature plants with small leaves; however, the underlying mechanism is unknown. Here, we investigated the small leaf phenotype of nup1–1 plants and found that cell number and size are reduced. Next, gene expression analysis revealed significant changes in the expression of several cell-cycle and expansion-related genes in leaves of nup1–1 plants compared to the wild-type control (Col-0). Furthermore, the subcellular localization of NUP1 throughout mitosis uncovered the potential role of NUP1 in aligning the chromosome during metaphase and separation of chromosomes in anaphase. Our findings suggest that NUP1 is required for maintaining normal plant stature by regulating cell size and number. Further protein-protein interaction of NUP1 and metaphase-anaphase-related proteins would help identify the precise roles of NUP1 in cell division.

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拟南芥核孔蛋白1参与细胞分裂和扩增

NUCLEOPORIN1 (NUP1)是核孔复合物的一个组成部分，也是TREX-2 mRNA输出复合物的一个锚点，此前有报道称在拟南芥中具有多种功能。几项研究表明，NUP1基因的突变导致植株矮小，叶片小;然而，其潜在机制尚不清楚。我们对nup1-1植株的小叶表型进行了研究，发现细胞数量和大小都减少了。接下来，基因表达分析显示，与野生型对照(Col-0)相比，nup1-1植株叶片中几个细胞周期和扩增相关基因的表达发生了显著变化。此外，NUP1在有丝分裂过程中的亚细胞定位揭示了NUP1在中期染色体排列和后期染色体分离中的潜在作用。我们的研究结果表明，NUP1是通过调节细胞大小和数量来维持正常植物高度所必需的。进一步研究NUP1与中期-后期相关蛋白的相互作用将有助于确定NUP1在细胞分裂中的确切作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Gene Agricultural and Biological Sciences-Plant Science

CiteScore

4.50

自引率

0.00%

发文量

审稿时长

51 days

期刊介绍： Plant Gene publishes papers that focus on the regulation, expression, function and evolution of genes in plants, algae and other photosynthesizing organisms (e.g., cyanobacteria), and plant-associated microorganisms. Plant Gene strives to be a diverse plant journal and topics in multiple fields will be considered for publication. Although not limited to the following, some general topics include: Gene discovery and characterization, Gene regulation in response to environmental stress (e.g., salinity, drought, etc.), Genetic effects of transposable elements, Genetic control of secondary metabolic pathways and metabolic enzymes. Herbal Medicine - regulation and medicinal properties of plant products, Plant hormonal signaling, Plant evolutionary genetics, molecular evolution, population genetics, and phylogenetics, Profiling of plant gene expression and genetic variation, Plant-microbe interactions (e.g., influence of endophytes on gene expression; horizontal gene transfer studies; etc.), Agricultural genetics - biotechnology and crop improvement.