Investigating the Relation between LCK Gene Expression with Type 2 Diabetes Patients in Yazd Diabetes Research Center

Abstract

Objective: Type 2 diabetes mellitus (T2DM) is characterized by insulin resistance and insulin secretory defect. Deficiency of cellular immunity is known as one of the factors involved in the pathogenesis of T2DM. lymphocyte-specific protein tyrosine kinase( LCK) is an important gene involved in the intracellular signaling pathways of lymphocytes. This study aimed at determining and comparing LCK gene expression levels in diabetic patients compared with the healthy controls. Materials and Methods: In this case-control study, 60 people, including 30 T2DM and 30 healthy people were included. The expression levels of the LCK gene were measured by real-time polymerase chain reaction and the obtained data were analyzed by T-test in GraphPad Prism6 software. Results: The expression level of the LCK gene was increased in diabetic samples compared with the healthy samples (P= 0.0001). Conclusion: The results suggested that changes in the expression levels of LCK gene can play a role in the pathogenesis of T2DM.