Marcadores str’s autosómicos en población indígena añu zuliana de Venezuela

Abstract

The state of Zulia concentrates 61.18% of the aboriginal population of Venezuela, of wich the Anu are the second largest group, after the Wayuu. 29 Anu individuals were studied, and dna extraction was performed by means of a method that combines two techniques (Fenol / Sevag and Salting-Out), proceeding to their amplification through multiplex pcr, using the Promega Powerplex®16HS Kit, determining the allele frequencies of 15 autosomal STR markers: D3S1358, TH01, D21S11, D18S51, PENTA E, D5S818, D13S317, D7S820, D16S539, CSF1P0, PENTA D, vWA, D8S1179, TPOX, FGA and AMELOGENINE. Genotyping was performed after the comparison of STR fragments with the allelic ladder and the standard internal lane size using the GeneMapper® ID-X software, Version 1.2, with the highest allelic frequencies for each genetic marker being the following: the allele 18 of the locus D3S1358 (0.448), allele 6 of TH01 (0.586), allele 30 D21S11 (0.379), allele 12 D18S51 (0.275), allele 14 Penta E (0.258), allele 11 D5S818 (0.603), allele 12 D13S317 (0.327) , allele 11 D7S820 (0.586), allele 12 D16S539 (0.310), allele 10 CSF1PO (0.344), allele 11 Penta D (0.327), allele 16 vWA (0.620), allele 14 D8S1179 (0.431), allele 8 TPOX (0.551) and allele 24 FGA (0.396). According to Nei’s matrix of distance, the Anu population does not share its genetic stock with other Zulian indigenous groups or the American continent, indicating that this ethnic group has its own ancestral origin with little or no external genetic contribution.