Effect on growth and productivity of lutein from the chlorophyta microalga, strain MCH of Muriellopsis sp., when grown in sea water and outdoor conditions at the Atacama Desert

IF 2.3 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Electronic Journal of Biotechnology Pub Date : 2021-11-01 DOI:10.1016/j.ejbt.2021.09.001

Victoria Cruz-Balladares , Paola Marticorena , Carlos Riquelme

{"title":"Effect on growth and productivity of lutein from the chlorophyta microalga, strain MCH of Muriellopsis sp., when grown in sea water and outdoor conditions at the Atacama Desert","authors":"Victoria Cruz-Balladares , Paola Marticorena , Carlos Riquelme","doi":"10.1016/j.ejbt.2021.09.001","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Microalgae are microorganisms that produce various products, for example, pigments, mainly carotenoids. This study aimed to used the strain of <em>Muriellopsis</em> sp. and to evaluate their behavior when grown in freshwater and seawater, along with indoor and outdoor conditions for both cultures. Growth of the strain was evaluated by determining its biomass, lutein productivity with high-performance liquid chromatography (HPLC), and antioxidant activity by using the 2,2-diphenyl-1-picrilhydrazil (DPPH method).</p></div><div><h3>Results</h3><p><em>Muriellopsis</em> sp. strain in indoor cultures showed an increased antioxidant activity. In outdoor conditions, both cultures showed increased cells number, concentration of biomass, and lutein productivity. The percentage of lutein obtained from the strain MCH in indoor conditions was 25 times higher than that reported for calendula, reaching 0.75% of lutein in <em>Muriellopsis</em> sp. cultured in seawater, followed by 0.6% in <em>Muriellopsis</em> sp., cultures in freshwater at day 12 of both cultures. These values exceed that of microalgae <em>Scenedesmus almeriensis</em>, which reaches 0.53% lutein.</p></div><div><h3>Conclusions</h3><p>The results show that the native strain of the Atacama Desert is one of the largest producers of lutein as compared to those reported to date. The study demonstrated the feasibility of producing this carotenoid with well-known properties to prevent some diseases due to its high nutritional value. <em>Muriellopsis</em> sp. cultivation in open-air seawater is a good precedent for developing mass production of this species in an area where freshwater is scarce and costly.</p><p><strong>How to cite</strong>: Cruz-Balladares V, Marticorena P, Riquelme C. Effect on growth and productivity of lutein from the chlorophyta microalga, strain MCH of <em>Muriellopsis</em> sp., when grown in sea water and outdoor conditions at the Atacama Desert. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.001</p></div>","PeriodicalId":11529,"journal":{"name":"Electronic Journal of Biotechnology","volume":"54 ","pages":"Pages 77-85"},"PeriodicalIF":2.3000,"publicationDate":"2021-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0717345821000440/pdfft?md5=13f2f6ec012e1e7ba879d42963e1178c&pid=1-s2.0-S0717345821000440-main.pdf","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Electronic Journal of Biotechnology","FirstCategoryId":"5","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0717345821000440","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 7

Abstract

Background

Microalgae are microorganisms that produce various products, for example, pigments, mainly carotenoids. This study aimed to used the strain of Muriellopsis sp. and to evaluate their behavior when grown in freshwater and seawater, along with indoor and outdoor conditions for both cultures. Growth of the strain was evaluated by determining its biomass, lutein productivity with high-performance liquid chromatography (HPLC), and antioxidant activity by using the 2,2-diphenyl-1-picrilhydrazil (DPPH method).

Results

Muriellopsis sp. strain in indoor cultures showed an increased antioxidant activity. In outdoor conditions, both cultures showed increased cells number, concentration of biomass, and lutein productivity. The percentage of lutein obtained from the strain MCH in indoor conditions was 25 times higher than that reported for calendula, reaching 0.75% of lutein in Muriellopsis sp. cultured in seawater, followed by 0.6% in Muriellopsis sp., cultures in freshwater at day 12 of both cultures. These values exceed that of microalgae Scenedesmus almeriensis, which reaches 0.53% lutein.

Conclusions

The results show that the native strain of the Atacama Desert is one of the largest producers of lutein as compared to those reported to date. The study demonstrated the feasibility of producing this carotenoid with well-known properties to prevent some diseases due to its high nutritional value. Muriellopsis sp. cultivation in open-air seawater is a good precedent for developing mass production of this species in an area where freshwater is scarce and costly.

How to cite: Cruz-Balladares V, Marticorena P, Riquelme C. Effect on growth and productivity of lutein from the chlorophyta microalga, strain MCH of Muriellopsis sp., when grown in sea water and outdoor conditions at the Atacama Desert. Electron J Biotechnol 2021;54. https://doi.org/10.1016/j.ejbt.2021.09.001

Abstract Image

查看原文本刊更多论文

在阿塔卡马沙漠海水和室外条件下生长对绿藻MCH菌株叶黄素生长和产量的影响

微藻是一种生产各种产品的微生物，例如色素，主要是类胡萝卜素。本研究旨在使用Muriellopsis sp.菌株，并评估它们在淡水和海水中生长时的行为，以及两种培养物的室内和室外条件。采用高效液相色谱法(HPLC)测定菌株的生物量、叶黄素产量和2,2-二苯基-1-吡咯肼(DPPH)法测定菌株的抗氧化活性，评价菌株的生长情况。结果室内培养菌抗氧化活性明显增强。在室外条件下，两种培养都显示出细胞数量、生物量浓度和叶黄素产量的增加。MCH菌株在室内条件下获得的叶黄素百分比比报道的金盏花高25倍，海水培养的Muriellopsis sp.的叶黄素含量达到0.75%，淡水培养的Muriellopsis sp.的叶黄素含量在12 d时达到0.6%。这些数值超过了微藻(Scenedesmus almeriensis)的叶黄素0.53%。结论与目前报道的叶黄素产量相比，阿塔卡马沙漠的原生菌株是叶黄素产量最大的菌株之一。该研究证明了生产这种类胡萝卜素的可行性，由于其高营养价值，它具有众所周知的预防某些疾病的特性。露天海水养殖是在淡水稀缺和成本昂贵的地区大规模生产该物种的良好先例。如何引用:Cruz-Balladares V, Marticorena P, Riquelme C. Muriellopsis .的绿藻菌株MCH在阿塔卡马沙漠海水和室外条件下生长对叶黄素生长和生产力的影响。中国生物医学工程学报(英文版);2011;16。https://doi.org/10.1016/j.ejbt.2021.09.001

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Electronic Journal of Biotechnology 工程技术-生物工程与应用微生物

CiteScore

5.60

自引率

0.00%

发文量

审稿时长

2 months

期刊介绍： Electronic Journal of Biotechnology is an international scientific electronic journal, which publishes papers from all areas related to Biotechnology. It covers from molecular biology and the chemistry of biological processes to aquatic and earth environmental aspects, computational applications, policy and ethical issues directly related to Biotechnology. The journal provides an effective way to publish research and review articles and short communications, video material, animation sequences and 3D are also accepted to support and enhance articles. The articles will be examined by a scientific committee and anonymous evaluators and published every two months in HTML and PDF formats (January 15th , March 15th, May 15th, July 15th, September 15th, November 15th). The following areas are covered in the Journal: • Animal Biotechnology • Biofilms • Bioinformatics • Biomedicine • Biopolicies of International Cooperation • Biosafety • Biotechnology Industry • Biotechnology of Human Disorders • Chemical Engineering • Environmental Biotechnology • Food Biotechnology • Marine Biotechnology • Microbial Biotechnology • Molecular Biology and Genetics •Nanobiotechnology • Omics • Plant Biotechnology • Process Biotechnology • Process Chemistry and Technology • Tissue Engineering