Methylobacterium sp. isolated from the midgut of Anopheles stephensi inhibits egg maturation in host ovary

IF 1.3 4区农林科学 Q2 ENTOMOLOGY

Applied Entomology and Zoology Pub Date : 2023-08-19 DOI:10.1007/s13355-023-00842-9

Jun Hakozaki, Yusuke Nonogaki, Taishi Tanabe, Keita Nishiyama, Kazuhiko Nakayama, Asako Haraguchi, Sakure Nakamura, Kodai Kusakisako, Hiromi Ikadai

{"title":"Methylobacterium sp. isolated from the midgut of Anopheles stephensi inhibits egg maturation in host ovary","authors":"Jun Hakozaki, Yusuke Nonogaki, Taishi Tanabe, Keita Nishiyama, Kazuhiko Nakayama, Asako Haraguchi, Sakure Nakamura, Kodai Kusakisako, Hiromi Ikadai","doi":"10.1007/s13355-023-00842-9","DOIUrl":null,"url":null,"abstract":"<div>The gut bacteria of insects are associated with digestion, nutrition, and reproduction. The midgut bacteria of Anopheles mosquitoes have been examined predominantly for their effects on the transmission of Plasmodium parasites. However, the effects of midgut bacteria on Anopheles mosquitoes have not been studied. In this study, we investigated the effects of midgut bacteria on adult female An. stephensi mosquitoes. These mosquitoes were fed antibiotics mixed with 10% sucrose for 2 weeks to alter the composition of their midgut bacteria. The results showed that the mosquitoes fed with streptomycin (ST) or kanamycin (KM) showed inhibited egg maturation 2 days after blood feeding. Methylobacterium sp. was the predominant bacterium in the midgut of mosquitoes fed KM or ST for 2 weeks (ST/2 weeks). Mosquitoes that were fed suspensions of Methylobacterium sp. isolated from the midgut of ST/2 weeks after blood feeding for 3 days showed inhibited egg maturation. Furthermore, egg maturation was inhibited in mosquitoes that were fed supernatant and precipitate obtained by boiling and centrifuging Methylobacterium sp. suspension. Thus, this study demonstrated that Methylobacterium sp. isolated from the midgut of An. stephensi mosquitoes after blood feeding inhibited egg maturation.</div>","PeriodicalId":8551,"journal":{"name":"Applied Entomology and Zoology","volume":"58 4","pages":"369 - 377"},"PeriodicalIF":1.3000,"publicationDate":"2023-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Entomology and Zoology","FirstCategoryId":"97","ListUrlMain":"https://link.springer.com/article/10.1007/s13355-023-00842-9","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"ENTOMOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

The gut bacteria of insects are associated with digestion, nutrition, and reproduction. The midgut bacteria of Anopheles mosquitoes have been examined predominantly for their effects on the transmission of Plasmodium parasites. However, the effects of midgut bacteria on Anopheles mosquitoes have not been studied. In this study, we investigated the effects of midgut bacteria on adult female An. stephensi mosquitoes. These mosquitoes were fed antibiotics mixed with 10% sucrose for 2 weeks to alter the composition of their midgut bacteria. The results showed that the mosquitoes fed with streptomycin (ST) or kanamycin (KM) showed inhibited egg maturation 2 days after blood feeding. Methylobacterium sp. was the predominant bacterium in the midgut of mosquitoes fed KM or ST for 2 weeks (ST/2 weeks). Mosquitoes that were fed suspensions of Methylobacterium sp. isolated from the midgut of ST/2 weeks after blood feeding for 3 days showed inhibited egg maturation. Furthermore, egg maturation was inhibited in mosquitoes that were fed supernatant and precipitate obtained by boiling and centrifuging Methylobacterium sp. suspension. Thus, this study demonstrated that Methylobacterium sp. isolated from the midgut of An. stephensi mosquitoes after blood feeding inhibited egg maturation.

Abstract Image

查看原文本刊更多论文

从斯氏按蚊中肠分离的甲基杆菌抑制宿主卵巢卵成熟

昆虫的肠道细菌与消化、营养和繁殖有关。研究按蚊的中肠细菌主要是为了研究它们对疟原虫传播的影响。然而，中肠细菌对按蚊的影响尚未得到研究。在本研究中，我们研究了中肠细菌对成年雌性安的影响。stephensi蚊子。这些蚊子被喂食含有10%蔗糖的抗生素2周，以改变其中肠细菌的组成。结果表明，链霉素(ST)和卡那霉素(KM)对采血后2 d的蚊卵成熟均有抑制作用。饲喂KM或ST 2周后，中肠细菌以甲基杆菌为主(ST/2周)。经血喂养2周后，饲喂ST中肠分离的甲基杆菌悬浮液3 d，蚊虫卵成熟受到抑制。另外，用甲基杆菌悬浮液煮沸和离心后得到的上清液和沉淀物喂蚊，可抑制卵的成熟。因此，本研究证实了从安家鸡中肠分离的甲基杆菌属。吸血后的斯氏蚊卵成熟受到抑制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Applied Entomology and Zoology 生物-昆虫学

CiteScore

2.70

自引率

7.70%

发文量

审稿时长

6-12 weeks

期刊介绍： Applied Entomology and Zoology publishes articles concerned with applied entomology, applied zoology, agricultural chemicals and pest control in English. Contributions of a basic and fundamental nature may be accepted at the discretion of the Editor. Manuscripts of original research papers, technical notes and reviews are accepted for consideration. No manuscript that has been published elsewhere will be accepted for publication.