LC-HRMS profiling of phytochemicals, antidiabetic, anticholinergic and antioxidant activities of evaporated ethanol extract of Astragalus brachycalyx Fischer

IF 1.8 Q3 CHEMISTRY, ANALYTICAL

Journal of Chemical Metrology Pub Date : 2021-12-26 DOI:10.25135/jcm.62.2107.2155

I. Gülçin, Hatice Kiziltaş, Zeynebe Bi̇ngöl, A. Gören, S. M. Pinar, S. Alwasel

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引用次数: 15

Abstract

Astragalus is a perennial plant that has existed for about 2500-3000 years and consists of more than 250 taxonomic parts. Twenty species of Astragalus are endemic to Turkey, as well as the richest genus with 425 taxa. The roots of Astragalus species are used in folk medicine as hepatoprotective, antioxidant, antibacterial, antihypertensive, antidiabetic and diuretic. Also, it is used to treat diabetes mellitus, leukemia, nephritis and uterine cancer. It is known that in Anatolia, Astragalus roots are traditionally used against leukemia and wound healing. For the purpose, the measuring of antioxidant activity of evaporated ethanol extract of Astragalus brachycalyx FISCHER (EEAB), some bioanalytic methods including DPPH and ABTS scavenging effects, ferric ions (Fe) and cupric ions (Cu) reducing abilities, and metal (Fe) chelating activity were realized. α-Tocopherol, ascorbic acid, and BHT were used as the standard antioxidants. On the other hand, some phenolic compounds, which responsible for antioxidant activities of EEAB was determined by liquid chromatography-high resolution mass spectrometry (LC-HRMS). At the similar concentration, EEAB exhibited efficient antioxidant effects when compared to standard compounds. Additionally, EEAB showed IC50 values of 1.985 μg/mL toward acetylcholinesterase (AChE), 0.620 μg/mL on α-glycosidase and 0.306 μg/mL against α-amylase enzymes.

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LC-HRMS分析短枝黄芪蒸发乙醇提取物的植物化学物质、抗糖尿病、抗胆碱能和抗氧化活性

黄芪是一种多年生植物，已有2500-3000年的历史，由250多个分类部分组成。20种黄芪是土耳其的特有种，也是最丰富的属，有425个分类群。黄芪的根在民间医学中具有保肝、抗氧化、抗菌、降压、抗糖尿病和利尿的作用。此外，它还用于治疗糖尿病，白血病，肾炎和子宫癌症。众所周知，在安纳托利亚，黄芪根传统上用于治疗白血病和伤口愈合。为此，实现了短枝黄芪蒸发乙醇提取物（EEAB）抗氧化活性的测定，包括DPPH和ABTS的清除作用、铁离子（Fe）和铜离子（Cu）的还原能力以及金属（Fe）螯合活性等生物分析方法。α-生育酚、抗坏血酸和BHT作为标准抗氧化剂。另一方面，采用液相色谱-高分辨质谱法（LC-HRMS）测定了EEAB抗氧化活性的一些酚类化合物。在相似的浓度下，与标准化合物相比，EEAB表现出有效的抗氧化作用。此外，EEAB对乙酰胆碱酯酶（AChE）的IC50值为1.985μg/mL，对α-糖苷酶的IC50为0.620μg/mL。

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来源期刊

Journal of Chemical Metrology CHEMISTRY, ANALYTICAL-

CiteScore

2.30

自引率

15.40%

发文量