Combined effects of bacteriophage vB_SauM-515A1 and antibiotics on the Staphylococcus aureus clinical isolates

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL

Bulletin of Russian State Medical University Pub Date : 2022-10-01 DOI:10.24075/brsmu.2022.052

NK Abdraimova, M. Kornienko, D. Bespiatykh, NS Kuptsov, R. Gorodnichev, E. Shitikov

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Abstract

Currently, the search for new therapy options for infectious diseases caused by multidrug-resistant Staphylococcus aureus is a priority. Combining antibiotics with virulent (lytic) bacteriophages may be considered a viable alternative to conventional antibiotic therapy. The study was aimed to assess the combined effects of the lytic bacteriophage vB_SauM-515A1 of Herelleviridae family and antibiotics of various classes on the Staphylococcus aureus clinical strains. Strains (n = 4) belong to the clinically significant sequence types ST1, ST8, ST121 and are characterized by multidrug resistance. Efficiency of the combination use of two antibacterial agents was assessed by comparison of optical densities of the test samples and controls after 24 hrs. of incubation. Mutually enhancing activities of bacteriophage used in combination with oxacillin, tetracycline and linezolid were revealed, in contrast to the separate use of each agent. Efficiency generally increased with the selected optimum multiplicity of infection values. No antagonism was revealed when combining the phage with antibiotics. Thus, virulent bacteriophage vB_SauM515A1 can be considered as a possible auxiliary therapeutic agent for antimicrobial-resistant strains of Staphylococcus aureus.

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噬菌体vB_SauM-515A1联合抗生素对金黄色葡萄球菌临床分离株的影响

目前，寻找耐多药金黄色葡萄球菌引起的传染病的新治疗方案是一个优先事项。将抗生素与强毒(溶解)噬菌体结合可能被认为是传统抗生素治疗的可行替代方案。本研究旨在评价黑氏菌科裂解噬菌体vB_SauM-515A1与各类抗生素对金黄色葡萄球菌临床菌株的联合作用。菌株(n = 4)属于具有临床意义的序列型ST1、ST8、ST121，具有多药耐药特征。通过对比24 h后样品与对照的光密度，评价两种抗菌药联合使用的效果。孵化。与单独使用不同，噬菌体与奥西林、四环素和利奈唑胺联合使用时具有相互增强的活性。效率一般随着感染值最优多重数的选择而提高。噬菌体与抗生素联用无拮抗作用。因此，强毒噬菌体vB_SauM515A1可被认为是金黄色葡萄球菌耐药菌株的一种可能的辅助治疗剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bulletin of Russian State Medical University MEDICINE, GENERAL & INTERNAL-

CiteScore

0.80

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0.00%

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期刊介绍： Bulletin of Russian State Medical University (Bulletin of RSMU, ISSN Print 2500–1094, ISSN Online 2542–1204) is a peer-reviewed medical journal of Pirogov Russian National Research Medical University (Moscow, Russia). The original language of the journal is Russian (Vestnik Rossiyskogo Gosudarstvennogo Meditsinskogo Universiteta, Vestnik RGMU, ISSN Print 2070–7320, ISSN Online 2070–7339). Founded in 1994, it is issued once every two months publishing articles on clinical medicine and medical and biological sciences, first of all oncology, neurobiology, allergy and immunology, medical genetics, medical microbiology and infectious diseases. Every issue is thematic. Deadlines for manuscript submission are announced in advance. The number of publications on topics in spite of the issue topic is limited. The journal accepts only original articles submitted by their authors, including articles that present methods and techniques, clinical cases and opinions. Authors must guarantee that their work has not been previously published elsewhere in whole or in part and in other languages and is not under consideration by another scientific journal. The journal publishes only one review per issue; the review is ordered by the editors.