Non-targeted metabolomic evaluations during seed germination and seedling growth in Salicornia brachiata (Roxb.) under saline conditions

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-08-28 DOI:10.1016/j.aquabot.2023.103712

Preeti T. Jacob , Jigar A. Sutariya , Shahrukh A. Siddiqui , Darshita K. Pandya , Mangal S. Rathore

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Abstract

Experimental studies were conducted for metabolomic profiling during seed germination and seedling development in Salicornia brachaita under saline conditions. The results revealed accumulation of sucrose, mannose, glycerol, methionine, tryptophan, glycerol, protocathechoic acid, and mannonic acid in germinating seeds. Abundance of rhamnose, glucose, glutamine, fructose, ornithine, quininic acid, proline and ketoglutaric acid were recorded during emergence of radical (EoR) and cotyledonary stage (CS) at 50% strength of seawater (SW) salinity. Higher levels of myo-inositol, ethanolamine, isoleucine and talose at 48 hours (hrs) of imbibition, EoR and CS stages; while glycine, tyrosine and turanose were so at CS stage only. Under 200 mM NaCl, richness of stearic acid, quercetin, leucine, erythritol and psicose were noted at 48 hrs of imbibition followed by EoR stage. Fructose, ornithine, mannitol, asparagine, mallic acid, glucose and citric acid were abundant at EoR whereas aminobutanoic acid, hexanedioic acid and tyramine were so at CS stage. Among detected metabolites maximum number of metabolites showed hits with amioacyl-tRNA biosynthesis pathway and the amino acid biosynthesis pathway had maximum impact during seedling development. Role of metabolic pathways (including amino acid metabolism) and differential expression of genes related to these pathways are suggested in meeting the energy needs for varied biological activities during seed germination and subsequent seedling development in S. brachiata.

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盐水条件下臂海蓬种子萌发和幼苗生长过程中的非靶向代谢组学评价

对咸水条件下小海角种子萌发和幼苗发育的代谢组学特征进行了研究。结果表明，蔗糖、甘露糖、甘油、蛋氨酸、色氨酸、甘油、原儿茶酸和甘露酸在发芽种子中积累。在50%海水(SW)盐度条件下，鼠李糖、葡萄糖、谷氨酰胺、果糖、鸟氨酸、奎宁酸、脯氨酸和酮戊二酸的丰度在自由基出现(EoR)和子叶期(CS)期间被记录下来。在抑制、EoR和CS阶段48小时肌醇、乙醇胺、异亮氨酸和talose水平较高;而甘氨酸、酪氨酸和葡聚糖只有在CS期才有。在200 mM NaCl条件下，在渗吸48 h后的EoR阶段，测定了硬脂酸、槲皮素、亮氨酸、赤藓糖醇和甘油三酯的丰富度。在提高采收率阶段果糖、鸟氨酸、甘露醇、天冬酰胺、苹果酸、葡萄糖和柠檬酸含量较高，而在提高采收率阶段氨基丁酸、己二酸和酪胺含量较高。在检测到的代谢物中，与amioacyl-tRNA生物合成途径相匹配的代谢物数量最多，氨基酸生物合成途径对幼苗发育的影响最大。研究结果表明，在臂章草种子萌发和幼苗发育过程中，代谢途径(包括氨基酸代谢)及其相关基因的差异表达在满足不同生物活动的能量需求中发挥着重要作用。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.