EFFECT OF SOLVENTS ON PHYTOCHEMICAL PROFILING BY GC-Ms ANALYSIS AND In vitro CYTOTOXICITY OF BEE PROPOLIS AGAINST LEUKEMIA AND LUNG CANCER CELL LINES

Abstract

Propolis is well-known for its favourable biological properties, which are widely employed in the treatment of a variety of disorders. It is high in chemical components that are dependent on the sort of plant that bees may access. The chemical makeup of an Indian Propolis sample was investigated using GCMS, and 22 chemicals were discovered. The MTT assay was used to test the ethanol extract of Propolis against leukaemia and lung cancer cell lines in vitro. The propolis extract was incubated with cancer cell lines for 24 hours, cytotoxicity was assessed using a colorimetric method, and the IC50 value was obtained. The findings showed that the propolis extract tested had substantial cytotoxic action. The conclusion is that Indian Propolis has potential. The Indian Propolis was shown to be a possible natural source of chemopreventive agent. The chemical makeup of Indian Propolis suggests that it could be a viable source of novel bioactive chemicals, but the molecules responsible for biological activity must be identified.