New method for simultaneous determination of dissolved organic carbon and its stable carbon isotope ratio in liquid samples: environmental applications

IF 1.1 4区 环境科学与生态学 Q4 CHEMISTRY, INORGANIC & NUCLEAR
F. Rampazzo, Malgorzata M. Formalewicz, Umberto Traldi, Seta Noventa, C. Gion, Maria de Castro, Chris R Brodie, Francesca Tiozzo, N. Calace, D. Berto
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引用次数: 0

Abstract

ABSTRACT This study reports the development of an all-in-one elemental analyser isotope ratio mass spectrometry (EA-IRMS) system modified for simultaneous analysis of dissolved organic carbon (DOC) concentration and its stable carbon isotope footprint (δ 13CDOC) in aqueous samples. The method involves a quantitative oxidation of DOC in a 200 µL liquid sample to CO2, after sample acidification and stripping by nitrogen. The detection limit of the method for DOC quantification was 0.2 mg C/L with an analytical precision of 12 %. Uncertainty of stable isotope determinations was 2 % at 0.2 mg DOC/L, while decreasing to 0.3 % at 20 mg DOC/L. Quantitative oxidation of DOC in aqueous samples was validated by using ring test water samples and Deep Sea reference seawater. The method performances of isotope analysis were evaluated by analysing different isotopic standard solutions. The applicability of the method was tested through the analysis of different environmental types of water, showing that δ 13CDOC ranged from – 23.30 to –31.85 ‰, allowing to characterize samples of different environmental origin. The developed method offers several advantages including rapidity, use of small sample volumes and minimal sample pre-treatment, making it a valuable tool for routine DOC concentration measurements paired with isotopic characterization.
同时测定液体样品中溶解有机碳及其稳定碳同位素比的新方法:环境应用
摘要本研究报告了一种改进的一体式元素分析仪同位素比质谱(EA-IRMS)系统的开发,该系统用于同时分析水样品中的溶解有机碳(DOC)浓度及其稳定碳同位素足迹(δ13CDOC)。该方法包括在样品酸化和氮气汽提后,将200µL液体样品中的DOC定量氧化为CO2。DOC定量方法的检测限为0.2 mg C/L,分析精度为12 %. 稳定同位素测定的不确定度为2 % 在0.2 mg DOC/L时,同时降至0.3 % 以20 mg DOC/L。通过使用环试水样和深海参考海水验证了水样中DOC的定量氧化。通过对不同同位素标准溶液的分析,评价了同位素分析方法的性能。通过对不同环境类型水的分析,测试了该方法的适用性,结果表明δ13CDOC在-23.30至-31.85之间 ‰, 从而能够表征不同环境来源的样品。所开发的方法具有快速、使用小样品体积和最小样品预处理等优点,使其成为常规DOC浓度测量和同位素表征的宝贵工具。
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来源期刊
CiteScore
2.80
自引率
7.70%
发文量
21
审稿时长
3.0 months
期刊介绍: Isotopes in Environmental and Health Studies provides a unique platform for stable isotope studies in geological and life sciences, with emphasis on ecology. The international journal publishes original research papers, review articles, short communications, and book reviews relating to the following topics: -variations in natural isotope abundance (isotope ecology, isotope biochemistry, isotope hydrology, isotope geology) -stable isotope tracer techniques to follow the fate of certain substances in soil, water, plants, animals and in the human body -isotope effects and tracer theory linked with mathematical modelling -isotope measurement methods and equipment with respect to environmental and health research -diagnostic stable isotope application in medicine and in health studies -environmental sources of ionizing radiation and its effects on all living matter
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