Melioration of enzymatic ethanol production from alkali pre-treated paddy straw promoted by addition of surfactant

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biocatalysis and Biotransformation Pub Date : 2022-03-29 DOI:10.1080/10242422.2022.2055469

Rimple Chaudhary, Jyoti Kaushal, Gursharan Singh, A. Kaur, S. Arya

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引用次数: 5

Abstract

Abstract Along with the cellulase enzyme, xylanase plays an efficient role in the production of biofuel from agricultural wastes by degrading the xylan sugar present in the hemicellulose of cell wall. This study aims to improve the sugar production from biomass by the use of different enzymes with surfactant. The objective of this study is to compare sugar production and bioethanol production from sodium hydroxide along polyethylene glycol pre-treated paddy straw (Oryza sativa L.) with different combination of xylanase and cellulose enzymes along with lignin-degrading laccase enzyme. In results, 10.87 g/l of ethanol with saccharification of 64.51% ± 0.90 was obtained when xylanase and laccase were used, while 18.40 ± 0.56 g/l of ethanol with saccharification of 84.01%±1.09 was obtained when cellulase and laccase enzymes were used. Maximum bioethanol production was found to be 19.20 ± 0.26g/l, which was obtained by combination of xylanase, cellulase and laccase enzymes together at 37 °C after 36 h.

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表面活性剂对碱预处理稻草酶促乙醇生产的促进作用

摘要木聚糖酶与纤维素酶一起，通过降解细胞壁半纤维素中的木聚糖糖，在利用农业废弃物生产生物燃料方面发挥着有效的作用。本研究旨在通过使用不同的酶和表面活性剂来提高生物质的糖产量。本研究的目的是比较木聚糖酶和纤维素酶以及木质素降解漆酶的不同组合下，氢氧化钠沿着聚乙二醇预处理的稻草（Oryza sativa L.）生产糖和生物乙醇。结果为10.87 g/l乙醇，糖化率为64.51% ± 当使用木聚糖酶和漆酶时，获得0.90，而18.40 ± 0.56 当使用纤维素酶和漆酶时，可获得糖化率为84.01%±1.09的g/l乙醇。生物乙醇的最大产量为19.20 ± 0.26g/l，由木聚糖酶、纤维素酶和漆酶在37 36℃后 h。

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来源期刊

Biocatalysis and Biotransformation 生物-生化与分子生物学

CiteScore

4.40

自引率

5.60%

发文量

审稿时长

3 months

期刊介绍： Biocatalysis and Biotransformation publishes high quality research on the application of biological catalysts for the synthesis, interconversion or degradation of chemical species. Papers are published in the areas of: Mechanistic principles Kinetics and thermodynamics of biocatalytic processes Chemical or genetic modification of biocatalysts Developments in biocatalyst''s immobilization Activity and stability of biocatalysts in non-aqueous and multi-phasic environments, including the design of large scale biocatalytic processes Biomimetic systems Environmental applications of biocatalysis Metabolic engineering Types of articles published are; full-length original research articles, reviews, short communications on the application of biotransformations, and preliminary reports of novel catalytic activities.