Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-08-15 DOI:10.4308/hjb.30.6.1121-1131

D. F. Kusharyati, Oedjijono, T. D. Satwika, Dwiana Muflihah Yulianti, Afifah Mariana, Anwar Rovik

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引用次数: 0

Abstract

The mangrove ecosystem is unique because it is located between marine and land. Little research is exploring lactic acid bacteria (LAB) from mangrove ecosystems. The LAB LG71 isolate is successfully isolated from the sediment of mangrove ecosystems on the coast of Logending, Jawa Tengah (Indonesia). In this study, we aimed to know the effect of glucose supplementation on the growth of LG71 isolate, bacteriocin production, and its application in food preservation. The characterization results showed that the LG71 isolate is Lactobacillus sp. Interestingly, the LG71 isolate is catalase-positive since this character is rarely found in the LAB group. Supplementing 0.25% glucose to MRSB medium and an incubation time of 15 hours is the best treatment for producing Lactobacillus LG71 isolate cell biomass. A 2% concentration of crude extract of Lactobacillus LG71 bacteriocins is the best concentration against Salmonella typhi both during in vitro and in vivo tests in fish balls. The addition of glucose affects the production of Lactobacillus LG71 cell biomass, and the bacteriocin derived from Lactobacillus LG71 gives increased protection against S. typhi and offers an alternative for food preservation.

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从印尼红树林沉积物中分离的致细菌乳酸菌：生长优化、细菌素生产及其在食品保鲜中的应用

红树林生态系统是独一无二的，因为它位于海洋和陆地之间。对红树林生态系统中乳酸菌(LAB)的研究很少。LAB LG71分离物成功地从印度尼西亚爪哇登加Logending海岸红树林生态系统的沉积物中分离出来。在本研究中，我们旨在了解葡萄糖补充对LG71分离物生长、细菌素产生的影响及其在食品保鲜中的应用。鉴定结果表明，LG71分离物为Lactobacillus sp.。有趣的是，LG71分离物过氧化氢酶阳性，因为这种特性在LAB组中很少发现。在MRSB培养基中添加0.25%葡萄糖，培养时间为15小时，是产生LG71乳杆菌分离物细胞生物量的最佳处理。在鱼丸的体内和体外试验中，乳酸杆菌LG71菌素粗提物浓度为2%时，对伤寒沙门氏菌的杀伤效果最佳。葡萄糖的添加会影响LG71乳杆菌细胞生物量的产生，而从LG71乳杆菌中提取的细菌素可以增强对伤寒沙门氏菌的保护，并为食品保存提供了一种选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.