Combining Gut Microbiota Data with Network Pharmacology to Explore the Mechanism of Danggui Sini Decoction Action Against Blood Stasis Syndrome in Rats

IF 0.4 4区 医学 Q4 NUTRITION & DIETETICS
Fengfeng Yu, Xiaoli Huang, Jiayu Liang, Shuyu Guan
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引用次数: 0

Abstract

Danggui Sini decoction is a traditional Chinese medicine preparation commonly used in the treatment of blood stasis syndrome through a mechanism yet to be defined. Therefore, we have explored its mechanism of action by combining pharmacology and gut microbiota analysis. To this end, female Sprague-Dawley rats were divided into 5 groups, including the control group, the acute blood stasis group, the Danshen tablet-treated group, the low-dose Danggui Sini decoction group, and the high-dose Danggui Sini decoction group. Danggui Sini decoction led to a significant reduction in the changes in the whole blood viscosity and plasma viscosity of rats with acute blood stasis syndrome, improved the coagulation function and cardiopulmonary pathological injury, upregulated the plasma 6-keto prostaglandin F1α and endothelial nitric oxide synthase, and downregulated thromboxane B2 and endothelin-1. The high throughput 16S rRNA gene sequencing results showed that Danggui Sini decoction reduced the number of pathogenic proteobacteria, significantly reduced erysipelas and suzericella, and increased the beneficial bacteria Roche, effectively improving the status of intestinal flora disorder in acute blood stasis rats. The Spearman correlation analyses showed that there was a close correlation between intestinal flora distribution and changes in blood stasis-related biochemical indices. In addition, network pharmacological analysis showed that the epidermal growth factor receptor, tumor protein p53, signal transducer and activator of transcription 3, vascular endothelial growth factor A, and hypoxia-inducible factor 1-alpha were the core targets of Danggui Sini decoction in alleviation of blood stasis. Further genetic ontological and pathway enrichment analyses suggested that the therapeutic effect of Danggui Sini decoction on blood stasis involved the lipid and atherosclerosis pathways, advanced glycation end-product-receptor for advanced glycation end-product signaling pathways in diabetic complications, fluid shear stress and atherosclerosis pathways, and hypoxia-inducible factors, 1-alpha signaling pathway, phosphatidylinositol 3-kinase- protein kinase B signaling, and other pathways. These results are helpful in understanding the mechanism by which Danggui Sini decoction improves blood stasis and provide a reference for further research.
结合肠道微生物数据和网络药理学探讨当归四逆汤对大鼠血瘀证的作用机制
当归四逆汤是一种常用于治疗血瘀证的中药制剂,其作用机制尚不明确。因此,我们结合药理学和肠道微生物群分析,探讨了其作用机制。为此,将雌性Sprague-Dawley大鼠分为5组,包括对照组、急性血瘀组、丹参片治疗组、当归四逆汤小剂量组和当归四逆汤大剂量组。当归四逆汤可显著降低急性血瘀证大鼠全血粘度和血浆粘度的变化,改善凝血功能和心肺病理损伤,上调血浆6-酮前列腺素F1α和内皮一氧化氮合酶,下调血栓素B2和内皮素-1。高通量16S rRNA基因测序结果显示,当归四逆汤降低了致病性蛋白菌的数量,显著减少了丹毒和毒毒毒,增加了有益菌罗氏,有效改善了急性血瘀大鼠肠道菌群紊乱的状况。Spearman相关分析表明,肠道菌群分布与血瘀证相关生化指标的变化密切相关。此外,网络药理学分析表明,表皮生长因子受体、肿瘤蛋白p53、信号转导和转录激活因子3、血管内皮生长因子A和缺氧诱导因子1-α是当归四逆汤的核心活血靶点。进一步的遗传本体论和通路富集分析表明,当归四逆汤对血瘀证的治疗作用涉及脂质和动脉粥样硬化通路、糖尿病并发症中晚期糖基化终产物信号通路的晚期糖基化度终产物受体、流体剪切应力和动脉粥样硬化通路以及缺氧诱导因子,1-α信号通路、磷脂酰肌醇3-激酶-蛋白激酶B信号通路和其他通路。这些结果有助于理解当归四逆汤活血化瘀的作用机制,为进一步研究提供参考。
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来源期刊
CiteScore
1.10
自引率
0.00%
发文量
36
审稿时长
>12 weeks
期刊介绍: Current Topics in Nutraceutical Research is an international, interdisciplinary broad-based peer reviewed scientific journal for critical evaluation of research on chemistry, biology and therapeutic applications of nutraceuticals and functional foods. The major goal of this journal is to provide peer reviewed unbiased scientific data to the decision makers in the nutraceutical and food industry to help make informed choices about development of new products. To this end, the journal will publish two types of review articles. First, a review of preclinical research data coming largely from animal, cell culture and other experimental models. Such data will provide basis for future product development and/or human research initiatives. Second, a critical evaluation of current human experimental data to help market and deliver the product for medically proven use. This journal will also serve as a forum for nutritionists, internists, neurologists, psychiatrists, and all those interested in preventive medicine. The common denominator of all of the topic to be covered by the journal must include nutraceuticals and/functional food. The following is an example of some specific areas that may be of interest to the journal. i) Role of vitamins, minerals, antioxidants and phytonutrients on cardiovascular health, cancer, diabetes, ocular health, mental health, men’s health, women’s health, infant nutrition, ii) Role of herbals on human health, iii) Dietary supplements and sleep, iv) Components of diet that may have beneficial effect on human health, v) regulation of apoptosis and cell viability, vi) Isolation and characterization of bioactive components from functional foods, vii) Nutritional genomics, and viii) Nutritional proteomics.
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