Validated LC-MS/MS method for quantitation of solasodine in rat urine and feces: Blocking nonspecific adsorption

IF 1.7 4区化学 Q3 CHEMISTRY, ANALYTICAL

Acta Chromatographica Pub Date : 2022-09-22 DOI:10.1556/1326.2022.01079

T. Lu, Xiaohong Wang, Qi Zhang, Kun Liu, Tongxin Xu, Quande Wang, Pengfei Zhao, Zhongzhe Cheng

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引用次数: 0

Abstract

Solasodine, a steroidal alkaloid, is distributed extensively in Solanaceae plants with multiple biological activities such as neuroprotection, antineoplastic and anticonvulsant activities. However, there is little information about the excretion of intact solasodine in vivo. To investigate its excretion, a reliable LC-MS/MS method for quantitation solasodine in rat urine and feces was established and validated. Sample preparation was carried out by liquid-liquid extraction using MTBE as extractant. Moreover, rat urine was preconditioned with BSA, an anti-adsorptive additive, to prevent the nonspecific binding of solasodine to containers and tubes. The method was validated over the range of 4–2000 ng mL−1. The correlation coefficient (r 2) were all above 0.999. The intra- and inter-day precision and accuracy were within 16.9% and between −11.0 and 8.9%, respectively. The recovery of solasodine in urine and feces was in the range of 72.5–80.3 and 75.7–80.2%, respectively. IS-normalized matrix factor ranged from 0.94 to 1.12 with RSD% ≤4.02%. This method was successfully applied to the excretion study of solasodine following oral and intravenous administration.

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hplc -MS/MS法测定大鼠尿液和粪便中索拉索定的含量:阻断非特异性吸附

茄碱是一种广泛分布于茄科植物中的甾体生物碱，具有神经保护、抗肿瘤、抗惊厥等多种生物活性。然而，关于完整的索拉索定在体内排泄的信息很少。为了研究其排泄情况，建立了一种可靠的LC-MS/MS定量测定大鼠尿液和粪便中索拉索定的方法。以MTBE为萃取剂，采用液-液萃取法制备样品。此外，用抗吸附添加剂BSA预处理大鼠尿液，以防止索拉索定与容器和管的非特异性结合。该方法在4-2000 ng mL−1范围内验证。相关系数(r 2)均大于0.999。日内、日间精密度和准确度分别在16.9%以内和- 11.0 ~ 8.9%之间。尿液和粪便中索拉地定的回收率分别为72.5 ~ 80.3和75.7 ~ 80.2%。is归一化矩阵因子范围为0.94 ~ 1.12,RSD%≤4.02%。该方法成功地应用于索拉索定口服和静脉给药后的排泄研究。

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来源期刊

Acta Chromatographica 化学-分析化学

CiteScore

4.00

自引率

0.00%

发文量

审稿时长

2.3 months

期刊介绍： Acta Chromatographica Open Access Acta Chromatographica publishes peer-reviewed scientific articles on every field of chromatography, including theory of chromatography; progress in synthesis and characterization of new stationary phases; chromatography of organic, inorganic and complex compounds; enantioseparation and chromatography of chiral compounds; applications of chromatography in biology, pharmacy, medicine, and food analysis; environmental applications of chromatography; analytical and physico-chemical aspects of sample preparation for chromatography; hyphenated and combined techniques; chemometrics and its applications in separation science.