THYROID FUNCTION OF RATS WITH EXPERIMENTAL AIT AT AN EARLY PERIOD AFTER THE INJECTION OF BIOPREPARATIONS OF CORD BLOOD

Abstract

The development of new methods for the treatment of autoimmune thyroid disease is an actual problem of endocrinology. The aim of research was studying of the cell-free effect biological product of cord blood with immunomodulatory properties "Cryocell-Cryocord " on rats’ thyroid function with AIT simulated at an early period of the study. Simulation of autoimmune thyroiditis was carried out by immunization with a suboperatively isolated human thyroid antigen in combination completing Freund's adjuvant on 30 adult male rats weighing 130 - 150 g. Correction was carried out by parenteral ingection of the "Cryocell-Cryocord". Hormonal research (determination of the content of thyroid hormones and antibodies to thyroglobulin (TG-AT) were effected by using standard test kits for enzyme-linked immunosorbent assay at early stages after exposure (7 days, 1 month). Statistical maintained of the results was carried out using the methods of variation statistics. It was used normal distribution to unpaired Student's test to compare indicators. The difference was considered significant at p<0.05. It has been shown the biological preparation of cord blood "Cryocell - Cryocord" has a positive effect with induced autoimmune thyroiditis. It has been noted the normalizing result of the effected correction on the thyroid function of animals in the early period of the studying. It has been proved after the introduction of the biological preparation "Cryocell - Cryocord" in animals with experimental autoimmune thyroiditis observed the inhibition of the manifestations of autoimmune aggression and the normalization of the functional activity of the thyroid gland. These investigations can be the foundation for the development of a new approach to the treatment of patients.