Enhancing survival of Demodex folliculorum (Acari: Demodecidae) under in vitro condition: Effect of temperature and culture media

IF 0.7 Q4 ENTOMOLOGY

Persian Journal of Acarology Pub Date : 2021-10-15 DOI:10.22073/PJA.V10I4.69561

H. Maldonado, Dary Mendoza, G. Garavito, Martha Lizarazo, L. Escaf, E. Egea

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Abstract

Demodex spp. are ectoparasites that live on mammal's skin. Demodex folliculorum Simon is important in the pathogenesis of blepharitis and other inflammatory skin diseases; however, the in vitro culture has not been achieved yet. In this study, the effect of temperature and culture medium on the survival of D. folliculorum was determined. Adult mites were obtained directly from eyelashes of individuals with a clinical diagnosis of Demodex blepharitis. Temperature ranges from 15–25 ℃, and three culture media (RPMI-1640, RPMI-1640 supplemented with human serum, and RPMI-1640 supplemented with fetal bovine serum) were tested. Results showed significant differences in the survival time of D. folliculorum among different treatments. The maximum survival of mite was 15.20 ± 1.03 days with RPMI-1640 supplemented with human serum at 20 ℃. This result is the longest reported survival time of D. folliculorum under in vitro maintenance conditions. This research presents a refinement of the methods previously reported to develop an in vitro culture of D. folliculorum.

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提高毛囊蠕形螨(螨亚纲:蠕形螨科)体外存活率:温度和培养基的影响

蠕形螨是生活在哺乳动物皮肤上的体外寄生虫。毛囊蠕形螨Simon在睑缘炎和其他炎症性皮肤病的发病机制中很重要；然而，体外培养尚未实现。在本研究中，确定了温度和培养基对毛囊乳杆菌存活的影响。成年螨直接从临床诊断为蠕形螨睑缘炎的个体的睫毛中获得。温度范围为15–25℃，测试了三种培养基（RPMI-1640、补充人血清的RPMI-1640%和补充胎牛血清的RPMI-1640）。结果显示，不同治疗方法对毛囊乳杆菌的生存时间有显著差异。RPMI-1640在20℃时补充人血清，螨的最大存活时间为15.20±1.03天。这一结果是报道的毛囊乳杆菌在体外维持条件下存活时间最长的结果。这项研究对先前报道的体外培养毛囊乳杆菌的方法进行了改进。

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来源期刊

Persian Journal of Acarology Agricultural and Biological Sciences-Insect Science

CiteScore

1.60

自引率

30.80%

发文量

审稿时长

20 weeks

期刊介绍： Persian Journal of Acarology (PJA) is a peer-reviewed international journal of the Acarological Society of Iran for publication of high quality papers on any aspect of Acarology including mite and tick behavior, biochemistry, biology, control, ecology, evolution, morphology, physiology, systematics and taxonomy. All manuscripts will be subjected to peer review before acceptance.