Genetic Diversity of Gracilaria spp. in the Intertidal Zone on the South Coast of Yogyakarta, Indonesia Based on DNA Barcoding with rbcL Marker

Q3 Agricultural and Biological Sciences

HAYATI Journal of Biosciences Pub Date : 2023-07-01 DOI:10.4308/hjb.30.5.907-917

Feni Susanti, R. Adharini, Dini Wahyu Kartika Sari, E. Setyobudi

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引用次数: 0

Abstract

Gracilaria spp. is a commercial seaweed utilized in various food, pharmaceutical, and health industries. Due to the high plasticity of seaweed, morphological identification must be validated by molecular identification using DNA barcoding. This study aims to identify the genetic diversity of Gracilaria spp. based on DNA barcoding with an rbcL marker. Samples were collected from six beaches, i.e., Trenggole, Drini, Siung, Wediombo, Nguyahan, and Ngedan, from September-November 2021. The methods in this study were sampling, sample preservation, morphological and tissue observations, DNA extraction, PCR and electrophoresis, and data analysis. The results showed four species of seaweed based on the rbcL marker: Gracilaria sp., G. salicornia, G. edulis, and G. vieillardii. Based on 18 samples confirmed by phylogenetics, five different species were found, namely G. salicornia, G. edulis, G. vieillardii, Gracilaria sp. 1, and Gracilaria sp. 2 The last two species, i.e., Gracilaria sp. 1 and Gracilaria sp. 2, from the intertidal zone of the south coast of Yogyakarta may have never been reported to GenBank.

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基于rbcL标记的印尼日惹南海岸潮间带江蓠属遗传多样性研究

龙须菜是一种商业海藻，用于各种食品、制药和健康行业。由于海藻的可塑性很高，必须通过使用DNA条形码的分子鉴定来验证形态鉴定。本研究旨在通过rbcL标记的DNA条形码鉴定龙须菜的遗传多样性。从2021年9月至11月，从特伦戈尔、Drini、Siung、Wediombo、Nguyahan和Ngedan六个海滩采集了样本。本研究的方法包括取样、样品保存、形态学和组织学观察、DNA提取、PCR和电泳以及数据分析。结果表明，基于rbcL标记的海藻有四种：龙须菜（Gracilaria sp.）、水杨（G.saliconia）、紫丁香（G.edulis）和vieillardii。根据系统发育学证实的18个样本，发现了5个不同的物种，即盐角藻、杜氏藻、vieillardii藻、龙须菜1和龙须菜2。来自日惹南海岸潮间带的最后两个物种，即龙须菜1和龙须菜2，可能从未向GenBank报告过。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

HAYATI Journal of Biosciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

24 weeks

期刊介绍： HAYATI Journal of Biosciences (HAYATI J Biosci) is an international peer-reviewed and open access journal that publishes significant and important research from all area of biosciences fields such as biodiversity, biosystematics, ecology, physiology, behavior, genetics and biotechnology. All life forms, ranging from microbes, fungi, plants, animals, and human, including virus, are covered by HAYATI J Biosci. HAYATI J Biosci published by Department of Biology, Bogor Agricultural University, Indonesia and the Indonesian Society for Biology. We accept submission from all over the world. Our Editorial Board members are prominent and active international researchers in biosciences fields who ensure efficient, fair, and constructive peer-review process. All accepted articles will be published on payment of an article-processing charge, and will be freely available to all readers with worldwide visibility and coverage.