A Modified Standardized Method to Extract and Store Insect Hemolymph with Use of a Glass Capillary

Abstract

Abstract Hemolymph is the “blood” circulating in the entire insect body. Analysis of this fluid gives information about the condition of the insect. The most precise analyses are conducted on insects’ hemolymph suspended in 0.6% physiological saline (NaCl). Most current hemolymph extraction methods are either difficult or do not provide pure material, the contamination of collected insect “blood” can change results. This study aimed to develop a technique for extract hemolymph, both easy and without risk of contaminating derived material. The presented method is a modification of available ways to extract, store and transport hemolymph with the use of a glass capillary. For the development of this technique, adult worker honey bees (Apis mellifera L.) were used. The method required such basic equipment as a glass capillary, sterile tweezer, Eppendorf tube and physiological saline. The collected hemolymph were frozen and hence sent in a glass capillary to another laboratory for analysis.