Anti-Metastatic Activity of Tagitinin C from Tithonia diversifolia in a Xenograft Mouse Model of Hepatocellular Carcinoma

Livers Pub Date : 2022-12-01 DOI:10.3390/livers2040030

Chuan-Yi Lin, May-Hua Liao, Chi-Yu Yang, Chao-Kai Chang, Shih-Mei Hsu, C. Juang, H. Wen

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引用次数: 1

Abstract

Sesquiterpenoid tagitinin C, present in Tithonia diversifolia leaves, has been known to have anti-hepatoma properties. Therefore, we investigated the anti-metastatic potential of tagitinin C in xenograft models of hepatocellular carcinoma (HCC). We isolated tagitinin C from a methanolic extract of the leaves of T. diversifolia. HepG-2 and Huh 7 hepatoma cells were treated with tagitinin C, and cell viability, migration, and matrix metalloproteinase (MPP) activity were assessed using the 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyltetrazolium bromide assay, scratch migration assay, and MMP activity assay, respectively. We used magnetic resonance spectroscopy to determine the tumorigenicity of xenografts inoculated with Hep-G2 and Huh 7 cells. Tagitinin C was cytotoxic against Hep-G2 and Huh 7 cells, with IC50 values of 2.0 ± 0.1 µg/mL and 1.2 ± 0.1 µg/mL, respectively, and it showed an anti-metastatic effect in vitro. Additionally, MRS assays revealed that tagitinin C (15 g/mouse/day) reduced the tumorigenicity of Hep-G2 and Huh 7 cell xenografts. Tagitinin C demonstrated significant antitumor and anti-metastatic activity in the two human hepatoma cell lines. Tagitinin C might be used as an alternative or auxiliary therapy for the treatment of HCC, and its effect should be further investigated in clinical settings.

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鬼针草Tagitinin C在肝癌异种移植小鼠模型中的抗转移活性

千叶香叶中存在的倍半萜类tagitinin C具有抗肝癌的特性。因此，我们研究了tagitinin C在肝细胞癌（HCC）异种移植模型中的抗转移潜力。我们从三叶草（T.diversifolia）叶的甲醇提取物中分离出tagitinin C。用tagitininC处理HepG-2和Huh7肝癌细胞，并分别用3-（4,5-二甲基硫唑-2-基）-2,5-二苯基溴化四氮唑测定法、划痕迁移测定法和MMP活性测定法评估细胞活力、迁移和基质金属蛋白酶（MPP）活性。我们使用磁共振波谱来确定接种Hep-G2和Huh7细胞的异种移植物的致瘤性。Tagitinin C对Hep-G2和Huh 7细胞具有细胞毒性，IC50值分别为2.0±0.1µg/mL和1.2±0.1µg/mL，在体外显示出抗转移作用。此外，MRS测定显示，tagitinin C（15g/小鼠/天）降低了Hep-G2和Huh 7细胞异种移植物的致瘤性。Tagitinin C在两种人肝癌细胞系中显示出显著的抗肿瘤和抗转移活性。Tagitinin C可能被用作HCC的替代或辅助治疗，其效果应在临床环境中进一步研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Livers

CiteScore

2.00

自引率

0.00%

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