Modification of SPION nanocarriers for siRNA delivery: A therapeutic strategy against HIV infection

Abstract

and the effectiveness of the modified SPION in siRNA delivery to HEK293 cells was evaluated. Results: The optimal concentration (50 µg/mL) of the modified SPION-containing anti-tat siRNA (with a range size of 50-70 nm and average zeta potential of +25 mV) was significantly internalized into the cells and decreased the expression of HIV-1 tat, more than 80%. Moreover, the nanoparticles showed no considerable toxicity on the cells. Conclusion: SPION could be optimized as a probable RNA/vaccine delivery system into target cells. Therefore, this study offers a therapeutic strategy against HIV or other infectious diseases.