{"title":"Oral supplementation of selenium improves post-thaw sperm quality in Saanen bucks","authors":"K. Lukusa, J. Kabuba","doi":"10.4103/2305-0500.321126","DOIUrl":null,"url":null,"abstract":"Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.","PeriodicalId":8564,"journal":{"name":"Asian Pacific Journal of Reproduction","volume":"10 1","pages":"187 - 194"},"PeriodicalIF":0.5000,"publicationDate":"2021-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Asian Pacific Journal of Reproduction","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.4103/2305-0500.321126","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"REPRODUCTIVE BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To determine the effect of oral selenium supplementation and semen collection methods on various post thaw semen quality parameters in Saanen bucks. Methods: Sixteen healthy bucks were divided into two equal groups (n=8 each). The treatment group received selenium at 10-day intervals for three months. Sperm kinematic parameters, morphological parameters, mitochondrial membrane potential, plasma membrane functionality, and sperm DNA integrity were evaluated weekly pre and post-cryopreservation. Results: The mean percentages of the morphological abnormalities were significantly lower in the selenium-supplemented samples when semen was collected by using artificial vagina method (P<0.05). Proximal droplet defects were significantly lower in the selenium supplementation group when semen was collected by electro-ejaculation (P<0.05). Post-thaw sperm parameters such as total motility and progressive motility were significantly higher when semen was obtained by artificial vagina in the selenium-supplemented bucks compared to the electro-ejaculation and the control groups (P<0.05). The sperm kinematic parameters such as curvilinear velocity, average path velocity, and amplitude of lateral head displacement were significantly higher when semen was collected by artificial vagina in the selenium-treated bucks (P<0.05). The percentages of sperm with intact and functional plasma membrane and functional mitochondria were significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups (P<0.05). In vitro fertilizing potential was significantly higher in the selenium-supplemented samples collected with artificial vagina compared to the electro-ejaculation method and the control groups, respectively (P<0.05). Conclusions: Oral supplementation of selenium and artificial vagina semen collection improve post thaw sperm quality parameters of Saanen buck.
期刊介绍:
The journal will cover technical and clinical studies related to health, ethical and social issues in field of Gynecology and Obstetrics. Articles with clinical interest and implications will be given preference.