METHOD DEVELOPMENT AND VALIDATION OF FLURBIPROFEN SODIUM BY USING UPLC

Abstract

A simple, precise, and accurate ultra-performance liquid chromatography was developed for flurbiprofen sodium on Acquity BEHC18, (50 x 2.1 mm, 1.7 µm) using acetonitrile: water: glacial acetic acid as the mobile phase. The flow rate was 0.2 mL min-1 and effluent was monitored at 254 nm. The retention time for flurbiprofen sodium was found to be 2.3. Developed method was validated for precision, accuracy, linearity range, robustness and ruggedness as per ICH guideline