Chlorella sorokiniana’ nın İzolasyonu, Moleküler Tanılanması, Fototrofik, Miksotrofik Ve Heterotrofik Üretimi

Abstract

: Microalgae was isolated using dilution and pouring plate method from the sea water sample taken from the Gumuldur region of Izmir. The species was identified as Chlorella sp. through morphologic evaluation under light microscope. Microalgae DNA was isolated through molecular methods and 16S and 18S rRNA gene regions were amplified by means of PCR. As a result of sequencing and phylogenetic evaluation of the gene regions, it was determined that the microalgae was Chlorella sorokiniana . Different methods such as centrifuge washing, antibiotic treatment, growth on agar and single cell isolation used to obtain axenic C. sorokiniana yielded successful results. Biomass and specific growth rate of phototrophic C. sorokiniana (0.19 g L -1 and 0.78 days -1 , respectively), mixotrophic C. sorokiniana (0.31 g L -1 and 1.3 days -1 , respectively) and heterotrophic C. sorokiniana (0.6 g L -1 and 2.52 days -1 , respectively) were determined. According to the findings obtained, the biomass productivity of axenic C. sorokiniana under different culture conditions can be ordered as: heterotrophy > mixotrophy > phototrophy.