In vivo efficacy of silver nanoparticles against Syphacia muris infected laboratory Wistar rats.

Q3 Immunology and Microbiology
Journal of Parasitic Diseases Pub Date : 2023-12-01 Epub Date: 2023-08-05 DOI:10.1007/s12639-023-01607-5
Mona Fathi Fol, Salwa Abdel-Hamid Hamdi, Heba Ali Abdel-Rahman, Nesma Abbas Mostafa
{"title":"In vivo efficacy of silver nanoparticles against <i>Syphacia muris</i> infected laboratory Wistar rats.","authors":"Mona Fathi Fol, Salwa Abdel-Hamid Hamdi, Heba Ali Abdel-Rahman, Nesma Abbas Mostafa","doi":"10.1007/s12639-023-01607-5","DOIUrl":null,"url":null,"abstract":"<p><p>Helminth infections are a worldwide problem that affects both humans and animals in developing countries. The common pinworm <i>Syphacia muris</i> frequently infects lab rats and can obstruct the creation of unrelated biological experiments. The objective of this study was to examine the in vivo efficacy of silver nanoparticles against <i>S. muris</i> infected Wistar rats. Transmission electron microscopy and X-ray diffraction examinations of silver nanoparticles revealed highly pure polycrystals with a mean size of 4 nm. Rats were divided into group I, the control: received distilled water; groups II and III, the treated: received 2, 4 mg/kg b.w. of Ag NPs, respectively. At the end of the experimental period, all rats were euthanized and dissected for collecting worms. The surface topography of the recovered worms was displayed using light and scanning electron microscopy, and their physiological status was determined using oxidative stress biomarkers. The histological changes in the rat liver, kidney, and spleen were also examined. In the current study, Ag NPs administration revealed substantial alterations in worms collected from treated rats, including shrinkage of lips, peeling and rupture of body cuticles, and disruption of surface annulations. Also, induced a significant increase in malondialdehyde and nitric oxide levels, as well as a decrease in reduced glutathione, glutathione peroxidase and catalase levels compared to control group. Moreover, sections of treated rats' liver, kidney and spleen displayed normal cellular appearance. In conclusion, this is the first in vivo study to evaluate Ag NPs efficacy against <i>S. muris</i> in laboratory rats without significant toxicity.</p>","PeriodicalId":16664,"journal":{"name":"Journal of Parasitic Diseases","volume":" ","pages":"744-756"},"PeriodicalIF":0.0000,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10667209/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Parasitic Diseases","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1007/s12639-023-01607-5","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/8/5 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Immunology and Microbiology","Score":null,"Total":0}
引用次数: 0

Abstract

Helminth infections are a worldwide problem that affects both humans and animals in developing countries. The common pinworm Syphacia muris frequently infects lab rats and can obstruct the creation of unrelated biological experiments. The objective of this study was to examine the in vivo efficacy of silver nanoparticles against S. muris infected Wistar rats. Transmission electron microscopy and X-ray diffraction examinations of silver nanoparticles revealed highly pure polycrystals with a mean size of 4 nm. Rats were divided into group I, the control: received distilled water; groups II and III, the treated: received 2, 4 mg/kg b.w. of Ag NPs, respectively. At the end of the experimental period, all rats were euthanized and dissected for collecting worms. The surface topography of the recovered worms was displayed using light and scanning electron microscopy, and their physiological status was determined using oxidative stress biomarkers. The histological changes in the rat liver, kidney, and spleen were also examined. In the current study, Ag NPs administration revealed substantial alterations in worms collected from treated rats, including shrinkage of lips, peeling and rupture of body cuticles, and disruption of surface annulations. Also, induced a significant increase in malondialdehyde and nitric oxide levels, as well as a decrease in reduced glutathione, glutathione peroxidase and catalase levels compared to control group. Moreover, sections of treated rats' liver, kidney and spleen displayed normal cellular appearance. In conclusion, this is the first in vivo study to evaluate Ag NPs efficacy against S. muris in laboratory rats without significant toxicity.

银纳米粒子对鼠Syphacia muris感染的实验室Wistar大鼠的体内疗效
蠕虫感染是一个世界性的问题,影响着发展中国家的人类和动物。常见的蛲虫鼠梅毒经常感染实验室大鼠,并可能阻碍不相关的生物实验的进行。本研究的目的是研究银纳米颗粒对鼠链球菌感染Wistar大鼠的体内疗效。透射电子显微镜和x射线衍射检查显示,银纳米粒子的平均尺寸为4纳米,高纯度多晶。大鼠分为第一组,对照组:给予蒸馏水;II组和III组分别给予2、4 mg/kg b.w.的Ag NPs。实验结束后,对所有大鼠实施安乐死,解剖取虫。利用光镜和扫描电镜观察恢复后线虫的表面形貌,并利用氧化应激生物标志物测定其生理状态。同时观察大鼠肝、肾、脾的组织学变化。在目前的研究中,Ag NPs给药显示了从治疗大鼠身上收集的蠕虫的实质性变化,包括嘴唇收缩,身体角质层剥落和破裂,以及表面环的破坏。此外,与对照组相比,丙二醛和一氧化氮水平显著增加,还原型谷胱甘肽、谷胱甘肽过氧化物酶和过氧化氢酶水平下降。此外,大鼠肝、肾和脾切片显示正常的细胞外观。总之,这是第一个在实验室大鼠中评估Ag NPs对S. muris的有效性而没有明显毒性的体内研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Parasitic Diseases
Journal of Parasitic Diseases Immunology and Microbiology-Parasitology
CiteScore
2.60
自引率
0.00%
发文量
86
期刊介绍: The primary constituency of the Journal of Parasitic Diseases is parasitology. It publishes original research papers (pure, applied and clinical), which contribute significantly to any area of parasitology. Research papers on various aspects of cellular and molecular parasitology are welcome.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信